eEF1A1 anticorps (N-Term)

N° du produit ABIN7540532

N° du produit (Fournisseur): 200-901-mp6

Cet anticorps Poulet Polyclonal détecte spécifiquement eEF1A1 dans WB, ELISA et IHC. Il présente une réactivité envers Humain.

Aperçu rapide pour eEF1A1 anticorps (N-Term) (ABIN7540532)

Antigène: eEF1A1 (EEF1A1) (Eukaryotic Translation Elongation Factor 1 alpha 1 (EEF1A1))

Reactivité: Humain

Hôte: Poulet

Clonalité: Polyclonal

Conjugué: Cet anticorp eEF1A1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Détail du produit anti-eEF1A1 anticorps

Épitope: N-Term

N° du produit (Fournisseur): 200-901-mp6

Fournisseur: Rockland

Fonction: EEF1A1 Antibody

Réactivité croisée (Details): This affinity purified antibody is directed against human EEF1A1.

Purification: This product is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above.

Stérilité: Sterile filtered

Immunogène: Anti-EEF1A1 antibody was prepared from eggs of chickens laid after repeated immunizations with a synthetic peptide corresponding to a N-terminal portion of human EEF1A1 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Information d'application

Indications d'application:

ELISA_Dilution: 1:10,000 - 1:50,000

Immunohistochemistry_Dilution: 1:100

Western_Blot_Dilution: 5 μg/mL

Commentaires:

Anti-EEF1A1 Antibody has been tested in Western Blot and IHC.  Expect a band at ~48-50 kDa in western blot using appropriate tissues and lysates.  Positive control used: MCF-7 nuclear extract lysate in western blot and human kidney tissue in Immunohistochemistry.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.002 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.0

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur eEF1A1

Antigène: eEF1A1 (EEF1A1) (Eukaryotic Translation Elongation Factor 1 alpha 1 (EEF1A1))

Autre désignation: EEF1A1

Sujet: Chicken Anti-Eukaryotic Translation Elongation Factor 1 Alpha 1, Leukocyte Receptor Cluster (LRC) Member 7, Leukocyte Receptor Cluster Member 7, Eukaryotic Elongation Factor 1 A-1, Elongation Factor 1-Alpha 1, Elongation Factor Tu, EF-1-Alpha-1, EEF1A-1, EEF1A, LENG7, EF-Tu, EF1A, Eukaryotic Translation Elongation Factor 1 Alpha 1-Like 14, Glucocorticoid Receptor AF-1 Specific Elongation Factor, Translation Elongation Factor 1 Alpha 1-Like 14, Epididymis Secretory Sperm Binding Protein, Elongation Factor 1 Alpha Subunit, Prostate Tumor-Inducing Protein 1, Cervical Cancer Suppressor 3, CTCL Tumor Antigen, EF1a-Like Protein, HNGC:16303, GRAF-1EF, CCS-3, EE1A1, EEF-1, CCS3, PTI1,EEF1A1 (Eukaryotic Translation Elongation Factor 1 Alpha 1) protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. With PARP1 and TXK, forms a complex that acts as a T helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66 % of patients with Felty syndrome. Anti-EEF1A1 is useful for researchers interested in Eukaryotic Translation Elongation Factor 1 Alpha-1-Like 14, Cervical Cancer, and the Innate Immune system.

ID gène: 1915

NCBI Accession: NP_001393

UniProt: P68104