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EEF1A2 anticorps (C-Term)

Cet anticorps Chèvre Polyclonal détecte spécifiquement EEF1A2 dans WB, ELISA, IHC, IF et FM. Il présente une réactivité envers Humain.
Rockland
N° du produit ABIN7540568
N° du produit (Fournisseur): 600-101-ml6s

Aperçu rapide pour EEF1A2 anticorps (C-Term) (ABIN7540568)

Antigène

Voir toutes EEF1A2 Anticorps
EEF1A2 (Eukaryotic Translation Elongation Factor 1 alpha 2 (EEF1A2))

Reactivité

  • 33
  • 16
  • 6
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Humain

Hôte

  • 37
  • 2
  • 1
Chèvre

Clonalité

  • 39
  • 1
Polyclonal

Conjugué

  • 21
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp EEF1A2 est non-conjugé

Application

  • 40
  • 18
  • 14
  • 13
  • 13
  • 10
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Fluorescence Microscopy (FM)
  • Épitope

    • 16
    • 8
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term

    N° du produit (Fournisseur)

    600-101-ml6s

    Fournisseur

    Rockland

    Fonction

    EEF1A2 Antibody

    Réactivité croisée (Details)

    This affinity purified antibody is directed against human EEF1A2.

    Purification

    The product was affinity purified from monospecific antiserum by immunoaffinity purification.

    Stérilité

    Sterile filtered

    Immunogène

    Anti-EEF1A2 antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to a near C-terminal portion of human EEF1A2 conjugated to Keyhole Limpet Hemocyanin (KLH).

    Isotype

    IgG
  • Indications d'application

    ELISA_Dilution: 1:10,000-1:50,000

    Immunohistochemistry_Dilution: 1:200

    IF_Microscopy_Dilution: 15 μg/mL

    Western_Blot_Dilution: 1:1000

    Commentaires

    Anti-EEF1A2 Antibody has been tested in WB, IF, and IHC.  Expect a band at ~50.5 kDa in western blot using appropriate lysates.  Positive control used: human skeletal muscle lysate, MCF-7 nuclear extract lysate in WB, MCF-7 cells in IF, and human skeletal muscle tissue in IHC.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Stock

    -20 °C

    Stockage commentaire

    Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

    Date de péremption

    12 months
  • Antigène

    EEF1A2 (Eukaryotic Translation Elongation Factor 1 alpha 2 (EEF1A2))

    Autre désignation

    EEF1A2

    Sujet

    Goat Anti-EEF1A2 Antibody, Goat Anti-Eukaryotic Translation Elongation Factor 1 Alpha 2 Antibody, Eukaryotic Elongation Factor 1 A-2, EF-1-Alpha-2, Statin-S1, EEF1AL, STN 3, Elongation Factor 1-Alpha 2, Statin-Like, EEF1A-2, EIEE33, Statin, MRD38, EF1A, STNL, HS1,EEF1A2 (Eukaryotic Translation Elongation Factor 1 Alpha 2) protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. EEF1A2 encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 2) is expressed in brain, heart and skeletal muscle, and the other isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas. EEF1A2 may be associated with the development of ovarian cancer, along with Epileptic Encephalopathy, Early Infantile, 33 and Mental Retardation, Autosomal Dominant 38. Anti-EEF1A2 Antibody is useful for researchers interested in cancer research, translation factors, GTP binding, and GTPase activities.

    ID gène

    1917

    NCBI Accession

    NP_001949

    UniProt

    Q05639
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