NEUROD1 anticorps (N-Term)

N° du produit ABIN7540577

N° du produit (Fournisseur): 600-101-mn7s

Cet anticorps Chèvre Polyclonal détecte spécifiquement NEUROD1 dans WB et ELISA. Il présente une réactivité envers Humain.

Aperçu rapide pour NEUROD1 anticorps (N-Term) (ABIN7540577)

Antigène: NEUROD1 (Neuronal Differentiation 1 (NEUROD1))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp NEUROD1 est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-NEUROD1 anticorps

Épitope: N-Term

N° du produit (Fournisseur): 600-101-mn7s

Fournisseur: Rockland

Fonction: NEUROD1 Antibody

Réactivité croisée (Details): This affinity purified antibody is directed against human NEUROD1.

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Stérilité: Sterile filtered

Immunogène: Anti-NEUROD1 Antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to a N-terminal region of human Neurogenic differentiation factor 1 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Information d'application

Indications d'application:

ELISA_Dilution: 1:10,000 - 1:50,000

Western_Blot_Dilution: 5 μg/mL

Commentaires:

Anti-NEUROD1 Antibody has been tested in Western Blot. Expect a band at ~39.9 kDa in western blot using appropriate lysates or tissues. Positive control used rec. NEUROD1 protein or SCLC-21H whole cell lysates in WB.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détails sur NEUROD1

Antigène: NEUROD1 (Neuronal Differentiation 1 (NEUROD1))

Autre désignation: NEUROD1

Sujet: Goat Anti-Neuronal Differentiation 1 Antibody, Neurogenic Helix-Loop-Helix Protein NEUROD, Class A Basic Helix-Loop-Helix Protein 3, Neurogenic Differentiation Factor 1, Beta-Cell E-Box Transactivator 2, NEUROD, BHLHa3, Basic Helix-Loop-Helix Transcription Factor, Neurogenic Differentiation 1, NeuroD1, BHLHA3, NeuroD, BETA2, BHF-1, MODY6,NEUROD1 (Neuronal Differentiation 1) gene encodes a member of the NeuroD family of basic helix-loop-helix (bHLH) transcription factors. It acts as a transcriptional activator: mediates transcriptional activation by binding to E box-containing promoter consensus core sequences 5'-CANNTG-3'. NEUROD1 associates with the p300/CBP transcription coactivator complex to stimulate transcription of the secretin gene as well as the gene encoding the cyclin-dependent kinase inhibitor CDKN1A. Contributes to the regulation of several cell differentiation pathways, like those that promote the formation of early retinal ganglion cells, inner ear sensory neurons, granule cells forming either the cerebellum or the dentate gyrus cell layer of the hippocampus, endocrine islet cells of the pancreas and enteroendocrine cells of the small intestine. Together with PAX6 or SIX3, it is required for the regulation of amacrine cell fate specification. It is also required for dendrite morphogenesis and maintenance in the cerebellar cortex. NEUROD1 associates with chromatin to enhancer regulatory elements in genes encoding key transcriptional regulators of neurogenesis. Anti-NEUROD1 is useful for researchers interested in Maturity-onset diabetes of the young Type 6, Diabetes mellitus noninsulin-dependent, as well as, developmental biology, DNA-binding transcription factor activity, and protein heterodimerization activity.

ID gène: 4760

NCBI Accession: NP_002491

UniProt: Q13562

Pathways: Dopaminergic Neurogenesis, Hormone Transport, Carbohydrate Homeostasis