MECP2 anticorps (C-Term)

N° du produit ABIN7540619

N° du produit (Fournisseur): 600-401-mm0

Cet anticorps Lapin Polyclonal détecte spécifiquement MECP2 dans WB, ELISA et IHC. Il présente une réactivité envers Humain.

Aperçu rapide pour MECP2 anticorps (C-Term) (ABIN7540619)

Antigène: MECP2 (Methyl CpG Binding Protein 2 (MECP2))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MECP2 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Détail du produit anti-MECP2 anticorps

Épitope: C-Term

N° du produit (Fournisseur): 600-401-mm0

Fournisseur: Rockland

Fonction: MeCP2 Antibody

Réactivité croisée (Details): This affinity purified antibody is directed against human MeCP2.

Purification: The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Stérilité: Sterile filtered

Immunogène: Anti-MeCP2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to C-terminal portion of human MeCP2 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Information d'application

Indications d'application:

ELISA_Dilution: 1:10,000 - 1:15,000

Immunohistochemistry_Dilution: 1:200

Western_Blot_Dilution: 1:1000

Commentaires:

Suggested Applications: This antibody is not suitable for CHIP.
Anti-MeCP2 Antibody has been tested in ELISA, Western Blot, and Immunohistochemistry. Expect a band at ~52 kDa in western blot using appropriate lysates or tissues. Positive control used U2-OS, A431, human cerebellum tissue, and Chicken Spinal Cord in WB, human tonsil tissue in IHC.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur MECP2

Antigène: MECP2 (Methyl CpG Binding Protein 2 (MECP2))

Autre désignation: MECP2

Sujet: Rabbit Anti-Methyl-CpG Binding Protein 2 Antibody, Rabbit-Anti-MeCP2 Antibody, MeCp-2 Protein, Methyl CpG Binding Protein 2 (Rett Syndrome), Testis Tissue Sperm-Binding Protein Li 41a, Mental Retardation, X-Linked 16, Mental Retardation, X-Linked 79, Methyl-CpG-Binding Protein 2, Methyl CpG Binding Protein 2, Rett Syndrome, AUTSX3, MRXS13, MRXSL, MRX16, MRX79, MeCp2, PPMX, RTS, RTT, RS,MeCP2 (Methyl-CpG Binding Protein 2) is a chromosomal protein that binds to methylated DNA. DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. MECP2 mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. It binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC). Anti-MeCP2 Antibody is useful for researchers interested in Rett Syndromes, SIDS, Mental Retardation X-Linked Syndromic 13, and chromatin binding pathways.

ID gène: 4204

NCBI Accession: NP_001104262

UniProt: P51608

Pathways: Inositol Metabolic Process, Chromatin Binding, Synaptic Membrane