SREBF1 anticorps (N-Term)

N° du produit ABIN7540628

N° du produit (Fournisseur): 600-401-mn1s

Cet anticorps Lapin Polyclonal détecte spécifiquement SREBF1 dans WB et ELISA. Il présente une réactivité envers Humain.

Aperçu rapide pour SREBF1 anticorps (N-Term) (ABIN7540628)

Antigène: SREBF1 (Sterol Regulatory Element Binding Transcription Factor 1 (SREBF1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SREBF1 est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-SREBF1 anticorps

Épitope: N-Term

N° du produit (Fournisseur): 600-401-mn1s

Fournisseur: Rockland

Fonction: SREBP1 Antibody

Réactivité croisée (Details): This affinity purified antibody is directed against human SREBP1.

Purification: The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Stérilité: Sterile filtered

Immunogène: Anti-SREBP1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a N-terminal portion of human SREBP1 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Information d'application

Indications d'application:

ELISA_Dilution: 1:10,000 - 1:50,000

Western_Blot_Dilution: 5 μg/mL

Commentaires:

Anti-SREBP1 Antibody has been tested in ELISA and WB.  Expect reactivity with different human isoforms and a band at ~124.6, ~121.7, ~113.6, ~48.3kDa in western blot using appropriate tissue or lysates.  Positive control used: HeLa and A549 lysates in Western Blot.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détails sur SREBF1

Antigène: SREBF1 (Sterol Regulatory Element Binding Transcription Factor 1 (SREBF1))

Autre désignation: SREBF1

Sujet: Rabbit Anti-SREBP1 Antibody, Rabbit Anti-Sterol Regulatory Element Binding Transcription Factor 1 Antibody, Class D Basic Helix-Loop-Helix Protein 1, SREBP1, BHLHd1, Sterol Regulatory Element-Binding Transcription Factor 1, Sterol Regulatory Element-Binding Protein 1, SREBP-1, BHLHD1,SREBF1 (Sterol Regulatory Element Binding Transcription Factor 1) is a transcriptional activator required for lipid homeostasis. It regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway. It binds to the sterol regulatory element 1 (SRE-1) (5-ATCACCCCAC-3), which is a motif that is found in the promoter of the low-density lipoprotein receptor gene and other genes involved in sterol biosynthesis. SREBP1 has dual sequence specificity binding to both an E-box motif (5-ATCACGTGA-3) and to SRE-1 (5-ATCACCCCAC-3). Anti-SREBP1 Antibody is useful for researchers interested in Fatty Liver Disease, Smith-Magenis Syndrome, Adipogenesis, DNA binding transcription factor activity and chromatin binding.

ID gène: 6720

NCBI Accession: NP_001005291

UniProt: P36956

Pathways: AMPK Signaling, Caspase Cascade in Apoptosis, Negative Regulation of Hormone Secretion, Regulation of Lipid Metabolism by PPARalpha