T4 RNA Ligase anticorps

N° du produit ABIN7540667

N° du produit (Fournisseur): 200-401-mx4

Cet anticorps Lapin Polyclonal détecte spécifiquement dans ELISA. Il présente une réactivité envers Bacteriophage T4.

Aperçu rapide pour T4 RNA Ligase anticorps (ABIN7540667)

Antigène: T4 RNA Ligase

Reactivité: Bacteriophage T4

Hôte: Lapin

Clonalité: Polyclonal

Application: ELISA

Détail du produit

N° du produit (Fournisseur): 200-401-mx4

Fournisseur: Rockland

Fonction: T4 RNA Ligase Antibody

Réactivité croisée (Details): Protein A purified T4 RNA Ligase antibody is directed against T4 RNA Ligase (E.coli)

Stérilité: Sterile filtered

Immunogène: Anti-T4 RNA Ligase antibody was produced from whole rabbit serum produced by repeated immunizations with T4 Ligase isolated from E. coli.

Isotype: IgG

Information d'application

Indications d'application: Optional[Neutralization_Dilution]: 1:13,000-1:14,000

Commentaires:

This protein A purified Anti-T4 RNA Ligase antibody has been tested for use in ELISA.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 43.5 kDa in size corresponding to RNA Ligase by western blotting in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détail du antigène

Antigène: T4 RNA Ligase

Sujet: Enterobacteria phage T4, RNA ligase 1, polyribonucleotide synthase (ATP), ribonucleic ligase,The T4 RNA Ligase enzyme catalyzes the ligation of RNA strands with 3'-hydroxyl and 5'-phosphate termini, forming a phosphodiester and sealing certain types of single-strand breaks in RNA. This catalysis occurs by a three-step mechanism, starting with the activation of the enzyme by ATP, forming a phosphoramide bond between adenylate and a lysine residue. The adenylate group is then transferred to the 5'-phosphate terminus of the substrate, forming the capped structure 5'-(5'-diphosphoadenosine)-[RNA]. Finally, the enzyme catalyzes a nucleophilic attack of the 3'-OH terminus on the capped terminus, which results in the formation of the phosphodiester bond and release of the adenylate.

ID gène: 258717

NCBI Accession: NP_049839

UniProt: A0A7S9SW04