T7 RNA Polymerase anticorps

N° du produit ABIN7540668

N° du produit (Fournisseur): 200-401-mx5

L’anticorps anti-T7 RNA Polymerase Polyclonal Lapin est utilisé pour la détection de T7 RNA Polymerase dans des échantillons de Bacteriophage T7. Il a été validé pour ELISA et WB.

Aperçu rapide pour T7 RNA Polymerase anticorps (ABIN7540668)

Antigène: T7 RNA Polymerase

Reactivité: Bacteriophage T7

Hôte: Lapin

Clonalité: Polyclonal

Application: ELISA, Western Blotting (WB)

Détail du produit

N° du produit (Fournisseur): 200-401-mx5

Fournisseur: Rockland

Fonction: T7 RNA Polymerase Antibody

Réactivité croisée (Details): Protein A purified T7 RNA Polymerase antibody is directed against T7 Polymerase (E.coli)

Stérilité: Sterile filtered

Immunogène: Anti-T7 Polymerase antibody was produced from whole rabbit serum produced by repeated immunizations with T7 Polymerase isolated from E. coli.

Isotype: IgG

Information d'application

Indications d'application:

ELISA_Dilution: 1:11,000-1:13,000

Western_Blot_Dilution: 1.0 μg/mL

Commentaires:

This protein A purified Anti-T7 RNA Polymerase antibody has been tested for use in ELISA and Western Blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 98.9 kDa in size corresponding to T7 Polymerase by western blotting in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détail du antigène

Antigène: T7 RNA Polymerase

Sujet: Escherichia phage T7 (Bacteriophage T7), DNA-directed RNA polymerase, DNA-dependent RNA polymerase, RNA nucleotidyltransferase (DNA-directed),T7 RNA Polymerase is a highly processive DNA-dependent RNA polymerase that catalyzes the transcription of class II and class III viral genes. It recognizes a specific promoter sequence and enters first into an 'abortive phase' where very short transcripts are synthesized and released before proceeding to the processive transcription of long RNA chains. T7 RNA Polymerase unwinds the double-stranded DNA to expose the coding strand for templating. It participates in the initiation of viral DNA replication presumably by making primers accessible to the DNA polymerase, thus facilitating the DNA opening. Also, T7 RNA Polymerase plays a role in viral DNA packaging, probably by pausing the transcription at the right end of concatemer junction to allow packaging complex recruitment and beginning of the packaging process. In eukaryotes three forms of this enzyme have been distinguished on the basis of sensitivity of alpha-amanitin, and the type of RNA synthesized.

ID gène: 1261050

NCBI Accession: NP_041960

UniProt: P00573