SARS-CoV-2 NSP8 anticorps

N° du produit ABIN7540672

N° du produit (Fournisseur): 100-401-a53

Cet anticorps anti-SARS-CoV-2 NSP8 est un anticorps Lapin Polyclonal détectant SARS-CoV-2 NSP8 dans WB, IF, FM et IP. Adapté pour SARS Coronavirus (SARS-CoV). Ce Primary Antibody a été cité dans 1 publication.

Aperçu rapide pour SARS-CoV-2 NSP8 anticorps (ABIN7540672)

Antigène: SARS-CoV-2 NSP8 (NSP8) (SARS-CoV-2 Non-Structural Protein 8 (NSP8))

Reactivité: SARS Coronavirus (SARS-CoV)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SARS-CoV-2 NSP8 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Fluorescence Microscopy (FM), Immunoprecipitation (IP)

Détail du produit anti-SARS-CoV-2 NSP8 anticorps

N° du produit (Fournisseur): 100-401-a53

Fournisseur: Rockland

Fonction: Sars-Cov Nonstructural Protein 8 Antibody

Réactivité croisée (Details): This antibody is directed against SARS-Coronavirus nsp8 protein.

Purification: The product is neat antiserum.

Stérilité: Sterile filtered

Immunogène: This whole rabbit serum was produced by repeated immunizations with a purified His- tagged recombinant protein corresponding to full-length SARS-Coronavirus nsp8.

Isotype: IgG

Information d'application

Indications d'application:

Immunoprecipitation_Dilution: 1:60

IF_Microscopy_Dilution: 1:300

Western_Blot_Dilution: 1:1,000

Other: IMMUNOELECTRON MICROSCOPY 1:100

Commentaires:

This antibody has been tested for use in immunofluorescence microscopy, immunoelectron microscopy, immunoprecipitation and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 22 kDa in size corresponding to SARS-CoV nsp8 by western blotting in the appropriate cell lysate or extract. For immunofluorescence microscopy, Vero-E6 cells, grown on glass slides, were infected with SARS-CoV-Fr1 strain for 1 h at 37°C.  Infection occurred in PBS/DEAE/2% FCS followed by exchange to EMEM/25mM HEPES/2% FCS. Cells were fixed with PBS/3% PFA.  After washing fixed cells, antibody incubation was performed in PBS/5% FCS for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: None

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-SARS-CoV-2 NSP8 (ABIN7540672)

Shi, Yu, Li, Cui, Zhao, Wang, Wang, Zhang, Huang: "Expression Profile and Localization of SARS-CoV-2 Nonstructural Replicase Proteins in Infected Cells." dans: Microbiology spectrum, Vol. 10, Issue 4, pp. e0074422, (2022) (PubMed).

Détails sur SARS-CoV-2 NSP8

Antigène: SARS-CoV-2 NSP8 (NSP8) (SARS-CoV-2 Non-Structural Protein 8 (NSP8))

Autre désignation: SARS-CoV-2 NSP8

Sujet: Rabbit anti-Sars-Cov Nonstructural Protein 8 Antibody, Replicase polyprotein 1a, ORF1a polyprotein, nsp8,The nonstructural protein 8 (nsp8) is one of the SARS-Coronavirus replicase cleaving products, encoded by ORF1a. Nsp8 is thought to be part of the viral replication complex, which is associated with intracellular membranes.  No specific information on the function of nsp8 is available. Anti-SARS-CoV Nonstructural Protein 8 (nsp8) Antibody is useful for researchers interested in viral research.

ID gène: 1489680, 30124074

UniProt: P0C6U8