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Recombinant CD43 anticorps

Cet anticorps anti-CD43 est un anticorps Lapin Chimeric détectant CD43 dans FACS, WB et RIA. Adapté pour Rat.
N° du produit ABIN7543048

Aperçu rapide pour Recombinant CD43 anticorps (ABIN7543048)

Antigène

Voir toutes CD43 (SPN) Anticorps
CD43 (SPN) (Sialophorin (SPN))

Type d'anticorp

Recombinant Antibody

Reactivité

  • 154
  • 36
  • 17
  • 1
  • 1
Rat

Hôte

  • 92
  • 83
  • 27
Lapin

Clonalité

  • 133
  • 66
  • 3
Chimeric

Conjugué

  • 85
  • 18
  • 18
  • 17
  • 11
  • 6
  • 4
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp CD43 est non-conjugé

Application

  • 128
  • 115
  • 82
  • 55
  • 44
  • 30
  • 26
  • 13
  • 7
  • 6
  • 5
  • 5
  • 5
  • 4
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
Flow Cytometry (FACS), Western Blotting (WB), Radioimmunoassay (RIA)

Clone

OX-58
  • Fonction

    Anti-CD43 [OX-58], Rabbit IgG, kappa

    Specificité

    This antibody recognises rat leukosialin, a major cell-surface sialoglycoprotein of thymocytes and T lymphocytes. It is also expressed on neutrophils, bone marrow stem cells and B-lineage cells after activation. The specificity of the leukosialin epitope is largely determined by linear protein sequence, as indicated by the reaction of this antibody with the extracellular domain of leukosialian expressed in an unglycosylated form in E. coli. However, the sensitivity of this antibody to neuraminidase treatment of leukosialin indicates that glycosylation can modify epitope conformation or flexibility.

    Attributs du produit

    Original Species of Ab: Mouse

    Original Format of Ab: IgG2b

    Purification

    Protein A affinity purified

    Immunogène

    This antibody was prepared by immunizing BALB/c mice with rat thymocyte leukosialin and thymocytes.

    Isotype

    IgG kappa
  • Indications d'application

    This antibody binds to rat leukosialin, and can be used to label thymocytes and T lymphocytes in the lymph node cell population in flow cytometry analysis. This antibody has also been used, along with other anti-CD43 MABs, in a Western blot analysis to localize leukosialin epitopes.

    Commentaires

    This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.

    Restrictions

    For Research Use only
  • Concentration

    1 mg/mL

    Buffer

    PBS with 0.02 % Proclin 300.

    Agent conservateur

    ProClin

    Précaution d'utilisation

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
  • Antigène

    CD43 (SPN) (Sialophorin (SPN))

    Autre désignation

    CD43

    Sujet

    Leukosialin, Leukocyte sialoglycoprotein, Sialophorin, W3/13 antigen, OX 58, MRC OX 58

    UniProt

    P13838

    Pathways

    Regulation of Leukocyte Mediated Immunity
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