Recombinant beta Catenin anticorps

N° du produit ABIN7543129

Cet anticorps anti-beta Catenin Monoclonal Souris (Clone 15B8) (ABIN7543129) détecte spécifiquement beta Catenin dans WB, IHC, IF, FACS et EMSA. L’anticorps est réactif avec des échantillons de Humain, Souris, Rat, Boeuf (Vache), Poulet et Chien.

Aperçu rapide pour Recombinant beta Catenin anticorps (ABIN7543129)

Antigène: beta Catenin (CATNB) (Catenin, beta (CATNB))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Poulet, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp beta Catenin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Electrophoretic Mobility-Shift Assay (EMSA)

Clone: 15B8

Détail du produit anti-beta Catenin anticorps

Fonction: Anti-beta catenin [15B8], Mouse IgG1, kappa

Specificité:

This antibody recognizes beta catenin. It does not react with plakoglobin. β-Catenin plays a dual function in the cell including cell-cell adhesion and as a component of the Wnt (wingless) signaling pathway.

Application Notes original Clone: This antibody is recommended for the detection and analysis of beta-catenin via various methods, such as Western blot, flow cytometry and immunofluorescence of cells and tissues. For instance, the antibody 15B8 was utilized in labelling beta-catenin in oral mucosa samples via immunohistochemistry (Das et al., 2013). Another group used this antibody in the identification of beta-catenin in extracts from K562 cells via Western blots (Kotula et al., 2014). Furthermore, this antibody was applied during immunohistochemistry stainings of transverse sections taken through the midbrain region of 8-9ss embryos (Schiffmacher et al., 2016). Finally,beta-catenin is a very popular marker used to outline the cell membrane, this antibody might be utilized for such purposes (Zhang et al., 2017).

Réactivité croisée: Poulet, Boeuf (Vache), Chien, Souris, Rat

Purification: Protein A affinity purified

Immunogène: This antibody was raised by immunizing mice with a recombinant full length beta-catenin of chicken origin.

Isotype: IgG1, kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

OriginalSpeciesName: Mouse

OriginalFormat: IgG1

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur beta Catenin

Antigène: beta Catenin (CATNB) (Catenin, beta (CATNB))

Autre désignation: beta catenin

Sujet: Catenin beta-1, Beta-catenin, β-catenin, β catenin

UniProt: P35222

Pathways: Peptide Hormone Metabolism