Recombinant HLA-A2 anticorps

N° du produit ABIN7543208

Cet anticorps Souris Monoclonal détecte spécifiquement dans FACS, ELISA, IHC, IF et WB. Il présente une réactivité envers Humain.

Aperçu rapide pour Recombinant HLA-A2 anticorps (ABIN7543208)

Antigène: HLA-A2

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Flow Cytometry (FACS), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blotting (WB)

Clone: BB7-2

Détail du produit

Fonction: Anti-HLA-A2 [BB7.2], Mouse IgG2b, kappa

Specificité: This antibody binds the carboxy terminus of the α2-helix of class I MHC molecule and a turn on one of the underlying β-strands.

Attributs du produit:

Original Species of Ab: Mouse

Original Format of Ab: IgG2b

Purification: Protein A affinity purified

Immunogène: The original antibody was generated by immunizing BALB/c mice with papain solubilized HLA-A2 and B7 antigens.

Isotype: IgG2b kappa

Information d'application

Indications d'application: The binding specificity of BB7.2 towards HLA-A2 was assessed using complement-mediated cytotoxicity and cellular radio binding assay. This antibody is cytotoxic for peripheral blood leukocytes and lymphoid cell lines. The original IgG2b version of this antibody binds HLA-A2 positive cells with a binding or avidity constant of about 5 x 108 liter/mol (PMID:7035415). This antibody was used to detect HLA-A*0201-PR1 complexes using western blot and ELISA (PMID: 17493346). This antibody was also used to detect HLA-A2 complexed with pol, gag, and EBV peptides using ELISA (PMID:8562505). This antibody was used to screen human leucocyte antigen (HLA)-A2 haplotype-positive HBV patients using flow cytometry (PMID: 16108766). This antibody was also used for the immunofluorescent staining of HLA-A2.1 Molecules on the cell surface of human processing defective cell line (T2) (PMID:7684681). This antibody was used in a study involving the expression of HLA class molecules in head and neck squamous cell carcinoma (HNSCC) specimens by immunohistochemistry (PMID: 9328140).

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détail du antigène

Antigène: HLA-A2

Classe de substances: Influenza Protein

Sujet: HLA-A, Human leukocyte antigen A, HLA class I histocompatibility antigen, A-2 alpha chain, MHC class I antigen A-2 HLA-A, HLA class I histocompatibility antigen A-28 alpha chain, HLA class I histocompatibility antigen, A-28 alpha chain, MHC class I antigen A-28

UniProt: P04439