Recombinant PD-1 anticorps

N° du produit ABIN7543218

L’anticorps Rat Monoclonal anti-PD-1 a été validé pour FACS, WB, IHC et BP. Il convient pour détecter PD-1 dans des échantillons de Souris.

Aperçu rapide pour Recombinant PD-1 anticorps (ABIN7543218)

Antigène: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Type d'anticorp: Recombinant Antibody

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp PD-1 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Blocking Peptide (BP)

Clone: 29F-1A12

Détail du produit anti-PD-1 anticorps

Fonction: Anti-PD-1 [29F.1A12], Rat IgG2a, kappa

Specificité: This antibody recognizes an epitope on mouse PD-1.

Attributs du produit:

Original Species of Ab: Rat

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogène: The antibody was raised by immunizing rats with plasmid DNA containing PD-1.

Isotype: IgG2a kappa

Information d'application

Indications d'application: This antibody was used for detection of PD-1 in CHO cells using flow cytometry. The ability of the antibody to block PD-1 interaction with PD-L1 or PD-L2 on CHO cells in an in vitro blocking assay was tested, the clone blocked PD-L1 Ig and PD-L2 Ig binding at high concentration, but only blocked 35 % of PD-L1 Ig or PD-L2 Ig binding at 10 μg/mL. This antibody was also used for immunohistochemical studies of various mouse tissues, such as heart (Liang et al, 2003, pmid: 14515254). In vivo anti-tumor effect in terms of level of tumor growth reduction was studied, a significant tumor growth reduction was observed in the tumors from mice treated with the antibody (Tobias et al, 2020, pmid: 32528470). The antibody was used for the detection of PD-1 in EL4 cells using flow cytometry. The antibody was used for cross-blocking with the unconjugated version of the same clones and it was reported to prevent PD-1 from interacting with PD-L1 (Polesso et al, 2021, pmid: 33684223). The antibody was used as a positive control for CT26 tumor model, a murine model of colon cancer (Kaumaya ey al., pmid:33117602).

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur PD-1

Antigène: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Autre désignation: PD-1

Sujet: CD279, Programmed cell death protein 1, Protein PD-1, mPD-1

UniProt: Q02242

Pathways: Cancer Immune Checkpoints