Recombinant Liver Arginase anticorps

N° du produit ABIN7543254

L’anticorps Lapin Chimeric anti-Liver Arginase a été validé pour cryo-EM et Inh. Il convient pour détecter Liver Arginase dans des échantillons de Humain.

Aperçu rapide pour Recombinant Liver Arginase anticorps (ABIN7543254)

Antigène: Liver Arginase (ARG1) (Arginase, Liver (ARG1))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Chimeric

Conjugué: Cet anticorp Liver Arginase est non-conjugé

Application: Cryogenic electron microscopy (cryo-EM), Inhibition (Inh)

Clone: MAb1

Détail du produit anti-Liver Arginase anticorps

Fonction: Anti-Arginase 1 [mAb1], Rabbit IgG, kappa

Specificité: The antibody sterically blocks the arginase 1 active site near the exterior surface of the enzyme. Approximately 80 % of all complexes identified were composed of two arginase 1 trimers and three antibodies, forming a 2:3 complex, while the remaining 20 % of complexes were composed of two arginase 1 trimers and two antibodies, however, the epitope and paratope interactions between each antibody and arginase 1 monomers are identical. Arginase is a manganese-containing enzyme that catalyzes the hydrolysis of L-arginine to L-ornithine and urea, and modulates T-cell-mediated immune response.

Attributs du produit:

Original Species of Ab: Mouse

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogène: The antibody is a human antibody identified via yeast display technologies and isolated after panning against arginase 1.

Isotype: IgG kappa

Information d'application

Indications d'application: The structure and epitope mapping of the complex arginase:antibody was determined by cryo-electron microscopy. The antibody was assessed for its potency and extent of inhibition of human arginase using an assay that detects the formation of thioornithine from thioarginine. In this assay, as well as an LCMS assay, it was determined that the antibody could fully inhibit the enzymatic activity of arginase. To assess the kinetic mechanism of inhibition of the antibody, increasing fixed concentrations of antibody were tested across a range of thioarginine concentrations. The Ki for the antibody against human Arg1 was 3.3 ± 0.3 nM (Palte et al., 2021, PMID: 34326456).

Commentaires:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur Liver Arginase

Antigène: Liver Arginase (ARG1) (Arginase, Liver (ARG1))

Autre désignation: Arginase 1

Sujet: ARG1, Arginase-1, Liver-type arginase, Type I arginase

UniProt: P05089

Pathways: Cellular Response to Molecule of Bacterial Origin