Recombinant PSMA anticorps (Extracellular Domain)

N° du produit ABIN7543287

L’anticorps anti-PSMA Chimeric Souris est utilisé pour la détection de PSMA dans des échantillons de Humain. Il a été validé pour WB, IHC, ELISA, FACS, IF et IP.

Aperçu rapide pour Recombinant PSMA anticorps (Extracellular Domain) (ABIN7543287)

Antigène: PSMA (FOLH1) (Folate Hydrolase (Prostate-Specific Membrane Antigen) 1 (FOLH1))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Souris

Clonalité: Chimeric

Conjugué: Cet anticorp PSMA est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: J591

Détail du produit anti-PSMA anticorps

Épitope: Extracellular Domain

Fonction: Anti-PSMA [J591], Mouse IgG1, lambda

Specificité:

This antibody binds the extracellular domain of the human PSMA. Prostate-specific membrane antigen (PSMA) is a type II integral membrane glycoprotein belonging to the M28 peptidase family. It acts as a glutamate carboxypeptidase on different alternative substrate. It is highly expressed in cancerous cells and is used as an effective diagnostic and prognostic indicator of prostate cancer.

Application Notes original Clone: This antibody was used in the immunohistochemical analysis of PSMA expressed on renal, urothelial, colon, lung, breast carcinomas and metastatic adenocarcinoma of liver. This antibody can also immunoprecipitate PSMA from crude LNCaP lysates (PMID: 9288760). This antibody was also used for the identification of PSMA in a western blot assay. The epitope analysis of this antibody was done using ELISA. This antibody was also used in the immunofluorescence staining of on nonpermeabilized and permeabilized LNCaP cells (PMID: 9288760). This antibody was used in a flow cytometric study for enumeration of circulating prostate microparticles (PMPs), a type of extracellular vesicle (EV) released by prostate cancer present in seminal fluid, urine, and blood may represent a non-invasive means to identify and prioritize patients with intermediate and high risk of prostate cancer (PMID: 26814433). In a phase 1 trial in patients with advanced solid tumor malignancies this antibody showed acceptable toxicity and excellent targeting of known sites of metastases (PMID: 17290063).

Attributs du produit: This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format for improved compatibility with existing reagents assays and techniques.

Purification: Protein A affinity purified

Immunogène: The original antibody was generated by immunizing BALB/c mice with LNCaP cells or a primary culture of PCa epithelial cells.

Isotype: IgG1, lambda

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

OriginalSpeciesName: Mouse

OriginalFormat: IgG1

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur PSMA

Antigène: PSMA (FOLH1) (Folate Hydrolase (Prostate-Specific Membrane Antigen) 1 (FOLH1))

Autre désignation: PSMA

Sujet: PSM, FOLH1, FOLH, FGCP, GCPII, mGCP, NAALAD1, GIG27, Prostate specific membrane antigen, Glutamate carboxypeptidase 2, Cell growth-inhibiting gene 27 protein, Folate hydrolase 1, Folylpoly-gamma-glutamate carboxypeptidase, Glutamate carboxypeptidase II, Membrane glutamate carboxypeptidase, N-acetylated-alpha-linked acidic dipeptidase I, NAALADase I, Prostate-specific membrane antigen, Pteroylpoly-gamma-glutamate carboxypeptidase

UniProt: Q04609