Recombinant Keratin 5/6 anticorps (AA 488-590)

N° du produit ABIN7543311

L’anticorps anti-Keratin 5/6 Chimeric Lapin est utilisé pour la détection de Keratin 5/6 dans des échantillons de Humain. Il a été validé pour IHC et ELISA.

Aperçu rapide pour Recombinant Keratin 5/6 anticorps (AA 488-590) (ABIN7543311)

Antigène: Keratin 5/6 (KRT5/6)

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Chimeric

Conjugué: Cet anticorp Keratin 5/6 est non-conjugé

Application: Immunohistochemistry (IHC), ELISA

Clone: AbM58019-7H4

Détail du produit anti-Keratin 5/6 anticorps

Épitope: AA 488-590

Fonction: Anti-Cytokeratin 5/6 [AbM58019-7H4], Rabbit IgG, kappa

Specificité: This antibody is capable of binding human cytokeratin 5 and human cytokeratin 6. Cytokeratins are intermediate filaments found in the intracytoplasmic cytoskeleton of epithelial tissue. Cytokeratin 5/6 is often expressed in a basal cell layer of normal mammary ducts. Studies have also shown that CK5/6 can be used as a characteristic marker of basal-like breast cancer.

Attributs du produit:

Original Species of Ab: Mouse

Original Format of Ab: IgG2b

Purification: Protein A affinity purified

Immunogène: The original antibody was generated by immunizing 4-6 weeks old female ICR mice with recombinant protein comprising amino acids 488-590 of cytokeratin 5 in equal volume of Freund's incomplete adjuvant.

Isotype: IgG kappa

Information d'application

Indications d'application: The binding characterization of this antibody was done using ELISA. The original IgG2b antibody binds cytokeratin 5/6 with a high affinity constant of 3.84 x 10^(9) M^(-1). This antibody was also used in the immunohistochemical analysis of esophagus and esophageal squamous cell carcinoma. The immunohistochemical staining pattern of this antibody is similar to commercially available antibody D5/16B4 (CN113845592).

Commentaires:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format for improved compatibility with existing reagents assays and techniques.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur Keratin 5/6

Antigène: Keratin 5/6 (KRT5/6)

Autre désignation: Cytokeratin 5/6

Sujet: CK5/6, CK5, CK-5, K5 KRT5, Keratin, type II cytoskeletal 5, cytokeratin-5, 58 kDa cytokeratin, Type-II keratin Kb5, CGMCC NO 21982, D5/16B4

UniProt: P13647