Recombinant FAP anticorps

N° du produit ABIN7543358

Cet anticorps Lapin Chimeric détecte spécifiquement FAP dans WB, IP, FACS, in vitro et in vivo. Il présente une réactivité envers Souris.

Aperçu rapide pour Recombinant FAP anticorps (ABIN7543358)

Antigène: FAP (Fibroblast Activation Protein, alpha (FAP))

Type d'anticorp: Recombinant Antibody

Reactivité: Souris

Hôte: Lapin

Clonalité: Chimeric

Conjugué: Cet anticorp FAP est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS), In vitro Assay (in vitro), In vivo Studies (in vivo)

Clone: 73-3

Détail du produit anti-FAP anticorps

Fonction: Anti-FAP [73.3], Rabbit IgG, kappa

Specificité: The antibody is specific for fibroblast activation protein (FAP). FAP is a membrane protease that is highly expressed by cancer-associated fibroblasts (CAFs).

Attributs du produit:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogène: The original antibody was generated by immunizing FAP-null mice with FAP-expressing 3T3 cells.

Isotype: IgG kappa

Information d'application

Indications d'application: A second-generation retroviral CAR, consisting of the scFv fragment of the antibody, with CD8 stalk, human CD3ζ and 4-1BB domains, targeting mouse FAP was developed. The Fap-CAR-T cells killed FAP-expressing 3T3 target cells specifically. The FAP-CAR-T cells were tested in vivo in three different established models, showing that the growth of tumors was significantly reduced by 35-50 % following treatment. Further, the anti-FAP-CAR T cells demonstrated enhanced antitumor response when combined with a tumor vaccine. No significant toxicity was observed in the treated mice. (Wang et al., 2014, PMID: 24778279) FAP+ cells were identified using flow cytometry of lung tissue using the antibody (Kimura et al., 2019, PMID: 31188013). This antibody was used for detection of murine FAP expression on HT1080 cells by flow cytometry (de Sostoa et al. 2019, PMID: 30683154). Immunoprecipitation of FAP was performed on lung humogenate using this antibody. The antibody detected FAP by western blot analysis (Fan et al., 2016, PMID: 26663085).

Commentaires:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur FAP

Antigène: FAP (Fibroblast Activation Protein, alpha (FAP))

Autre désignation: FAP

Sujet: Fibroblast activation protein, Fibroblast activation protein-α, Prolyl endopeptidase FAP, Dipeptidyl peptidase FAP, Fibroblast activation protein alpha, FAPalpha1, Gelatine degradation protease FAP, Integral membrane serine protease, Post-proline cleaving enzyme,Serine integral membrane protease, SIMP, Surface-expressed protease, Seprase

UniProt: P97321

Pathways: Tube Formation