Recombinant F11R anticorps (Extracellular Domain)

N° du produit ABIN7543378

Cet anticorps anti-F11R Chimeric Lapin (Clone 6F4) (ABIN7543378) détecte spécifiquement F11R dans WB, IHC, ELISA, FACS et IF. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour Recombinant F11R anticorps (Extracellular Domain) (ABIN7543378)

Antigène: F11R (F11 Receptor (F11R))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Chimeric

Conjugué: Cet anticorp F11R est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 6F4

Détail du produit anti-F11R anticorps

Épitope: Extracellular Domain

Fonction: Anti-JAM-A [6F4], Rabbit IgG, kappa

Specificité:

This antibody specifically recognizes human junctional adhesion molecule A and does not cross-react with other members of the JAM family. JAM-A is an adherens and tight junction protein expressed by endothelial and epithelial cells. It is recently identified as an antigenic protein (Ag) overexpressed in tumor cells and associated with cancer progression.

Application Notes original Clone: This antibody was used for the characterization of human junctional adhesion molecule-A (JAM-A) and study of its expression on various cancerous tissues. The binding specificity of this antibody for human JAM-A expressed on CHO cells was confirmed using flow cytometry. JAM-A expression level on cell surface was determined by immunofluorescence staining and visualized by confocal microscopy. This antibody can also be used in the determination of JAM-A protein using western blot. Immunohistochemical analysis using this antibody revealed that JAM-A is overexpressed on breast, lung and kidney tumor tissues. Injections of anti-JAM-A antibody resulted in a significant tumor growth inhibition of xenograft human tumors in vivo. Treatment with monoclonal antibody induced a decrease of the Ki67 expression and downregulated JAM-A levels. This antigen may play a role in interfering with tumor proliferation (PMID: 22886345). This antibody binds the extracellular domain of JAM-A in an ELISA (Goetsch et al, 2011).

Attributs du produit: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Purification: Protein A affinity purified

Immunogène: The original antibody was generated by immunizing BALB/c mice with MCF-7 cells. The humanized version was generated by grafting CDRs of parental mouse antibody onto human framework regions.

Isotype: IgG, kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

OriginalSpeciesName: Mouse

OriginalFormat: IgG1

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur F11R

Antigène: F11R (F11 Receptor (F11R))

Autre désignation: JAM-A

Sujet: CD321, JAM-1, JAM1, JCAM, F11R, PAM-1, Junctional adhesion molecule A, Junctional adhesion molecule 1, Platelet F11 receptor

UniProt: Q9Y624

Pathways: Cell-Cell Junction Organization