Recombinant Laminin anticorps

N° du produit ABIN7543382

Cet anticorps Lapin Chimeric détecte spécifiquement Laminin dans ELISA, WB, IF et FACS. Il présente une réactivité avec des échantillons de Humain et Souris.

Aperçu rapide pour Recombinant Laminin anticorps (ABIN7543382)

Antigène: Laminin (LAMA2) (Laminin, alpha 2 (LAMA2))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Chimeric

Conjugué: Cet anticorp Laminin est non-conjugé

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: C3_Hu10

Détail du produit anti-Laminin anticorps

Fonction: Anti-Laminin-2 LG5 [C3_Hu10], Rabbit IgG, kappa

Specificité:

This antibody recognizes human and mouse laminin-2 LG5 and LG4/5 domain. Laminin is a large extracellular heterotrimeric glycoprotein and a major constituent of the basement membrane. It is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

Application Notes original Clone: The binding specificity of this antibody for human and mouse laminin-2 LG5 was confirmed using ELISA. This antibody is capable of detecting human and mouse LG-4/5 expressed on HEK293 in a flow cytometry. This antibody can also be used for the identification recombinant human laminin-2 (Hu 211), murine LG-5 (mLG5), human LG-5 (hLG5), and human LG-4/5 (hLG4/LG5) in a western blot. This antibody was also used for the immunofluorescent staining of unfixed human and mouse muscle tissues. This antibody was used in combination with anti-laminin-2 antibody AS30_Hu6 to generate a duobody called TBTI (US11254737).

Réactivité croisée: Souris

Attributs du produit: This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format for improved compatibility with existing reagents assays and techniques.

Purification: Protein A affinity purified

Immunogène: The original antibody was generated by immunizing Balb/c and Trianni mice with human laminin-2 LG5 domain. Humanized version was generated by grafting CDRs of parental mouse antibody C3 onto human framework regions.

Isotype: IgG, kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

OriginalSpeciesName: Human

OriginalFormat: IgG1

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur Laminin

Antigène: Laminin (LAMA2) (Laminin, alpha 2 (LAMA2))

Autre désignation: Laminin-2 LG5

Sujet: Merosin, Laminin subunit alpha-2, LAMA2, Laminin 211, laminin G-like 5 domain, LG5, g-like 4 and 5 domain, LG-4/5, Laminin M chain, Laminin-12 subunit alpha, Laminin-2 subunit alpha, Laminin-4 subunit alpha, Merosin heavy chain, C3, humanized C3

UniProt: P24043, Q60675