Recombinant PD-1 anticorps

N° du produit ABIN7543419

L’anticorps Souris Monoclonal anti-PD-1 a été validé pour FACS et ELISA. Il convient pour détecter PD-1 dans des échantillons de Humain.

Aperçu rapide pour Recombinant PD-1 anticorps (ABIN7543419)

Antigène: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PD-1 est non-conjugé

Application: Flow Cytometry (FACS), ELISA

Clone: 1G4

Détail du produit anti-PD-1 anticorps

Fonction: Anti-PD-1 [1G4], Mouse IgG1, kappa

Specificité: This antibody binds human PD-1 protein. PD-1 acts as an inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self. It delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2.

Réactivité croisée: Macaque rhésus

Attributs du produit:

Original Species of Ab: Mouse

Original Format of Ab: IgG4

Purification: Protein A affinity purified

Immunogène: The original antibody was generated by immunizing 6-8 weeks old female BALB/c mice with his-tagged human PD-1 protein.

Isotype: IgG1 kappa

Information d'application

Indications d'application: The initial binding characterization of the antibody was done using ELISA. The binding reactivity of this antibody to human, mouse and rhesus monkey PD-1 expressed on CHO cells was determined using flow cytometry. This antibody was shown to cross react with human and rhesus monkey PD-1 and not mouse PD-1. The binding affinity of the original mouse IgG4 antibody for human PD-1 was determined using surface plasmon resonance and was found to be Kd= 1.07 nM. The anti-tumor effect of this antibody was tested in vivo in a mouse colon cancer model. Murine MC-38 cells (colon adenocarcinoma cells) were injected subcutaneously in B-hPD-1 mice and treated with this antibody. It was reported that this antibody showed good tumor inhibitory effect (CN111763257).

Commentaires:

This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG format for improved compatibility with existing reagent assays and techniques.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur PD-1

Antigène: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Autre désignation: PD-1

Sujet: CD279, PD1, PD 1, hPD-1, PDCD1, Programmed cell death protein 1, Programmed cell death 1, Protein PD-1, 41-1G4, 1G4-mHvKv

UniProt: Q15116

Pathways: Cancer Immune Checkpoints