Recombinant FTH1 anticorps

N° du produit ABIN7543446

L’anticorps anti-FTH1 Monoclonal Souris est utilisé pour la détection de FTH1 dans des échantillons de Humain. Il a été validé pour WB, ELISA et IHC.

Aperçu rapide pour Recombinant FTH1 anticorps (ABIN7543446)

Antigène: FTH1 (Ferritin Heavy Chain 1 (FTH1))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp FTH1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Clone: 4C9

Détail du produit anti-FTH1 anticorps

Fonction: Anti-Ferritin H [4C9], Mouse IgG1, kappa

Specificité: This antibody binds human acidic isoferritin. Ferritin is a protein that catalyzes the oxidation of ferrous iron(II) to ferric iron(III) and stores iron in a soluble, non-toxic, readily available form. Ferritin is important for iron homeostasis. Ferritins occur as many isoforms, all of which are composed of various combinations of the two primary subunits, H and L. Acidic isoferritins have high proportions of H subunit.

Attributs du produit:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogène: The original antibody was generated by immunizing BALB/c mice with acidic isoferritin (AIF) from primary hepatic carcinoma (PHC) tissues.

Isotype: IgG1 kappa

Information d'application

Indications d'application: The binding specificity of this antibody towards acidic isoferritin, basic isoferritin and human liver ferritin was determined using ELISA. This antibody does not cross react with basic isoferritin and human liver ferritin. The binding affinity of the original mouse IgG1 format of this antibody for acidic isoferritin was reported to be Kd= 4.15 nM. This antibody was used for the immunohistochemical staining of liver tissues of primary hepatic carcinoma (PHC)(PMID: 11604110). This antibody can be used for the identification of acidic isoferritins in a western blot (PMID: 12697395).

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur FTH1

Antigène: FTH1 (Ferritin Heavy Chain 1 (FTH1))

Autre désignation: Ferritin H

Sujet: FTH1, FTHL6, AIF, acidic isoferritin, Ferritin heavy chain, Ferritin H subunit

UniProt: P02794

Pathways: Transition Metal Ion Homeostasis