Avidin anticorps (FITC)

N° du produit ABIN7565742

Cet anticorps anti-Avidin est un anticorps Lapin Polyclonal détectant Avidin dans WB, DB, FACS, FLISA et FM. Adapté pour Poulet.

Aperçu rapide pour Avidin anticorps (FITC) (ABIN7565742)

Antigène: Avidin (AVD)

Reactivité: Poulet

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Avidin est conjugé à/à la FITC

Application: Western Blotting (WB), Dot Blot (DB), Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)

Détail du produit anti-Avidin anticorps (FITC)

Fonction: Avidin Antibody Fluorescein Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum and Avidin.

Attributs du produit: Synonyms: rabbit anti-Avidin Antibody fluorescein Conjugation, FITC conjugated rabbit anti-Avidin antibody, Avidin fluorescein, Anti-Avidin FITC Antibody, egg ehite

Purification: Anti-Avidin Antibody Fluorescein Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogène:

Immunogen: Avidin (Hen Egg White)

Immunogen Type: Native Protein

Labeling Ratio: 3.0

Information d'application

Indications d'application: Application Note: Rabbit Anti-Avidin Fluorescein conjugate has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Flow Cytometry Dilution: User Optimized Western Blot Dilution: >1:1,000 FLISA Dilution: >1:1,000 IF Microscopy Dilution: User Optimized Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Buffer: Restore with deionized water (or equivalent)

Reconstitution Volume: 100 μL

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Détails sur Avidin

Antigène: Avidin (AVD)

Autre désignation: AVIDIN

Sujet: Background: Avidin is a glycoprotein with a molecular weight of approximately 62.4 kDa. Avidin is a biotin binding protein that shows high sequence homology in birds, reptiles and amphibians. Hen egg white avidin is a tetrameric protein composed of four identical subunits, each with the ability to bind biotin with high affinity and specificity (Kd ~ 1015 M). In biotechnology, the functional consequence of tetrameric biotin binding is signal amplification. Biotin-avidin bridging is a great way to increase signal strength while maintaining specificity. The sequence of avidin only shows 30 % homology with streptavidin, and anti-avidin and anti-streptavidin antibodies are not immunologically cross reactive.

UniProt: P02701