CPB1 anticorps (HRP)

N° du produit ABIN7565752

Cet anticorps Lapin Polyclonal détecte spécifiquement CPB1 dans WB et ELISA. Il présente une réactivité envers Porc.

Aperçu rapide pour CPB1 anticorps (HRP) (ABIN7565752)

Antigène: CPB1 (Carboxypeptidase B1 (Tissue) (CPB1))

Reactivité: Porc

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CPB1 est conjugé à/à la HRP

Application: Western Blotting (WB), ELISA

Détail du produit anti-CPB1 anticorps (HRP)

Fonction: Carboxypeptidase B Antibody HRP Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Carboxypeptidase B [Porcine Pancreas].

Attributs du produit: Synonyms: rabbit anti-Carboxypeptidase B antibody HRP conjugation, peroxidase conjugated rabbit anti-Carboxypeptidase B Antibody, CarboxypeptidaseB1 antibody, CP B1 antibody, CPB 1 antibody, CPB antibody, CPB1 antibody, DKFZp779K1333 antibody, Pancreas specific protein antibody, Pancreatic carboxypeptidase B1 antibody

Purification: Anti-Carboxypeptidase B Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Carboxypeptidase B [Porcine Pancreas]

Immunogen Type: Native Protein

Information d'application

Indications d'application: Application Note: Anti-Carboxypeptidase B peroxidase Antibody has been tested in western blot and suitable to be assayed against 1.0 μg of Carboxypeptidase B [Porcine Pancreas] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:3,000 of the reconstitution concentration is suggested for Anti-Carboxypeptidase B Antibody. Western Blot Dilution: 1:250 - 1:1,000 ELISA Dilution: 1:1,000 - 1:5,000 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détails sur CPB1

Antigène: CPB1 (Carboxypeptidase B1 (Tissue) (CPB1))

Autre désignation: CPB1

Sujet: Background: Anti-Carboxypeptidase B Antibody is a key enzyme in the hydrolysis in basic amino acids in the C-terminal position of polypeptides. First isolated from the porcine pancreas, Carboxypeptidase B is activated by trypsin and exhibits high specificity for lysine and arginine. Carboxypeptidase B is also a key enzyme in insulin product in humans and can be used as a marker for pancreatic diagnosis. Anti-Carboxypeptidase B is ideal for investigators in Cell Biology, Immunology, and Microbiology.

ID gène: 397341

NCBI Accession: NP_999334

UniProt: P09955