Choline Oxidase anticorps (HRP)

N° du produit ABIN7565753

L’anticorps Chèvre Polyclonal anti-Choline Oxidase a été validé pour WB, ELISA et IP. Il convient pour détecter Choline Oxidase dans des échantillons de Alcaligenes.

Aperçu rapide pour Choline Oxidase anticorps (HRP) (ABIN7565753)

Antigène: Choline Oxidase

Reactivité: Alcaligenes

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Choline Oxidase est conjugé à/à la HRP

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Détail du produit anti-Choline Oxidase anticorps (HRP)

Fonction: Choline Oxidase Antibody Peroxidase Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Choline Oxidase [Alcaligenes species].

Attributs du produit: Synonyms: goat anti-Choline Oxidase Antibody HRP Conjugation, Peroxidase Conjugated goat anti-Choline Oxidase Antibody, codA antibody

Purification: Anti-Choline oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Choline Oxidase [Alcaligenes species]

Immunogen Type: Native Protein

Information d'application

Indications d'application: Application Note: Anti-Choline oxidase has been tested by ELISA and western blot and is assayed against 1.0 μg of Choline Oxidase [Alcaligenes species] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product. Western Blot Dilution: 1:500 - 1:1,000 Immunoprecipitation Dilution: 1:100 ELISA Dilution: 1:1,000 - 1:2,500 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détails sur Choline Oxidase

Antigène: Choline Oxidase

Sujet: Background: Choline oxidase is an enzyme that catalyzes the chemical reaction (choline + O2 <=> betaine aldehyde + H2O2). Thus, the two substrates of this enzyme are choline and O2, whereas its two products are betaine aldehyde and H2O2. Choline oxidase belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. This enzyme participates in glycine, serine, and threonine metabolism.

ID gène: 116367

UniProt: P16101