FTH1 anticorps (HRP)

N° du produit ABIN7565761

Cet anticorps Lapin Polyclonal détecte spécifiquement FTH1 dans WB, IHC, ELISA et FM. Il présente une réactivité envers Humain.

Aperçu rapide pour FTH1 anticorps (HRP) (ABIN7565761)

Antigène: FTH1 (Ferritin, Heavy Polypeptide 1 (FTH1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp FTH1 est conjugé à/à la HRP

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Fluorescence Microscopy (FM)

Détail du produit anti-FTH1 anticorps (HRP)

Fonction: Ferritin Antibody Peroxidase Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Ferritin [Human Spleen].

Attributs du produit: Synonyms: rabbit anti-Ferritin Antibody HRP Conjugation, peroxidase conjugated rabbit anti-Ferritin Antibody, FTHL6, Ferritin H subunit, Ferritin heavy chain like, Ferritin heavy polypeptide 1, Ferritin L subunit, Ferritin light chain like, Ferritin light polypeptide, Ferritin, heavy polypeptide, FTH

Purification: Anti-Ferritin (Human Spleen) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Ferritin [Human Spleen]

Immunogen Type: Native Protein

Information d'application

Indications d'application: Application Note: Anti-Ferritin (Human Spleen) Peroxidase Antibody has been tested by ELISA and western blot. This product is assayed against 1.0 μg of Ferritin [Human Spleen] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:12,000 to 1:60,000 of the reconstitution concentration is suggested for Anti-Ferritin (Human Spleen) Antibody. Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: User Optimized ELISA Dilution: User Optimized IF Microscopy Dilution: User Optimized Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détails sur FTH1

Antigène: FTH1 (Ferritin, Heavy Polypeptide 1 (FTH1))

Autre désignation: FTH1

Sujet: Background: Ferritin is a intracellular protein that directly stores iron and regulates its release, specifically acting as a buffer for iron in humans. Ferritin is composed of 24 subunits and comes in two types, H and L. The H subunit detoxifies iron while the L subunit deals with long term storage of iron. Depending on the concentration of ferritin in the blood, different disorders and diseases could be present in an individual. Anti-Ferritin (Human Spleen) Antibody is ideal for investigators in Cancer, Immunology, and Cardiovascular Research.

ID gène: 2495

UniProt: P02794

Pathways: Transition Metal Ion Homeostasis