HAO1 anticorps (HRP)

N° du produit ABIN7565768

L’anticorps Mouton Polyclonal anti-HAO1 a été validé pour WB et DB. Il convient pour détecter HAO1 dans des échantillons de Aspergillus niger.

Aperçu rapide pour HAO1 anticorps (HRP) (ABIN7565768)

Antigène: HAO1 (Hydroxyacid Oxidase (Glycolate Oxidase) 1 (HAO1))

Reactivité: Aspergillus niger

Hôte: Mouton

Clonalité: Polyclonal

Conjugué: Cet anticorp HAO1 est conjugé à/à la HRP

Application: Western Blotting (WB), Dot Blot (DB)

Détail du produit anti-HAO1 anticorps (HRP)

Fonction: Glucose Oxidase Antibody Peroxidase Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum as well as purified and partially purified Glucose Oxidase [Aspergillus niger].

Attributs du produit: Synonyms: sheep anti-Glucose Oxidase HRP conjugated Antibody, peroxidase conjugated sheep anti-Glucose Oxidase Antibody, Beta D Glucose Oxygen 1 Oxido Reductase antibody, Glucose oxidase (Precursor) antibody, Glucose Oxyhydrase antibody, GOD antibody

Purification: Anti-Glucose Oxidase (Sheep) antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Glucose Oxidase [Aspergillus niger]

Immunogen Type: Native Protein

Information d'application

Indications d'application: Application Note: Anti-Glucose Oxidase peroxidase conjugated antibody has been tested by dot blot and is suitable to be assayed against 1.0 μg of Glucose Oxidase [Aspergillus niger] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:3,000 of the reconstitution concentration is suggested for this product. Western Blot Dilution: User Optimized Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détails sur HAO1

Antigène: HAO1 (Hydroxyacid Oxidase (Glycolate Oxidase) 1 (HAO1))

Autre désignation: gox

Sujet: Background: Glucose oxidase is an enzyme that drives the oxidation of glucose into hydrogen peroxide and D-glucono-δ-lactone. This activity is utilized in biotechnology through colormetric detection assays that are sensitive to the amount of hydrogen peroxide produced. Horseradish Peroxidase (HRP) is an enzyme that utilizes organic peroxide compounds as electron donors. Horseradish Peroxidase naturally provides protection for plants against pathogens, but can be utilized in molecular biology to convert various substrates to detectable compounds (such as in Western Blotting and ELISAs). Anti-Glucose Oxidase (Sheep) antibody is ideal for investigators in Microbiology, Immunology, and Cell Biology research.

UniProt: P13006

Pathways: Monocarboxylic Acid Catabolic Process