HBd anticorps (N-Term)

N° du produit ABIN7565770

Cet anticorps Souris Monoclonal détecte spécifiquement HBd dans ELISA et WB. Il présente une réactivité envers Humain.

Aperçu rapide pour HBd anticorps (N-Term) (ABIN7565770)

Antigène: HBd (Hemoglobin, delta (HBd))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp HBd est non-conjugé

Application: ELISA, Western Blotting (WB)

Clone: 21G1-F1-B9-G9-D11

Détail du produit anti-HBd anticorps

Épitope: N-Term

Fonction: Hemoglobin beta A-2 Antibody

Réactivité croisée (Details): This protein A purified mouse monoclonal antibody reacts specifically with human HbA-2 delta isoform.

Attributs du produit: Synonyms: mouse anti-HbA-2 antibody, mouse anti-hemoglobin antibody, Hemoglobin Subunit Delta, Hemoglobin Delta Chain 4, Delta-Globin 4, HbD, HbA-2 Antibody, Sickle Cell Disease (SCD)

Purification: Anti-HbA-2 is purified from tissue culture supernatant by protein A purification.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Anti-Hemoglobin beta A-2 Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near the N-terminus of Hb delta-subunit conjugated to KLH.

Immunogen Type: Conjugated Peptide

Isotype: IgG1 kappa

Information d'application

Indications d'application: Application Note: Anti-Hemoglobin beta A-2 (MOUSE) antibody has been tested by ELISA and Western Blotting. This antibody is designed for use in lateral flow. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 16 kDa. Western Blot Dilution: 1 μg/mL ELISA Dilution: 1:20,000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.00 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détails sur HBd

Antigène: HBd (Hemoglobin, delta (HBd))

Autre désignation: HBD

Sujet: Background: HbA-2 or hemoglobin delta subunit antibodies detect the delta-specific sequence in the hemoglobin delta-subunit found in HbA-2. Functional hemoglobin (Hb) is a hetero tetramer and the dominant form of Adult Hb is composed of 2 alpha and 2 beta subunits (α2β2). Hemoglobin A-2 (HbA-2) is a normal but minor variant of hemoglobin A that consists of two alpha and two delta chains (α2δ2). Hemoglobin A-2 may be increased in beta thalassemia or in people who are heterozygous for the beta thalassemia gene, and HbA2 is also linked to neurological disorders. HbA-2 form exists in small amounts in all adult humans (1.5-3.1 % of all hemoglobin molecules) and is increased in people with Sickle-cell disease. Its normal biological role is not well understood. HbA-2 antibody does not react other forms of Hb including no cross-reaction to HbA or beta subunit. This antibody is ideal for investigators involved in Cardiovascular and developmental biology research.

ID gène: 3045

NCBI Accession: NP_000510

UniProt: P02042