Hemoglobin Subunit beta-C (LOC100134870) (N-Term) anticorps

N° du produit ABIN7565771

L’anticorps Souris Monoclonal anti- a été validé pour ELISA et WB. Il convient pour détecter dans des échantillons de Humain.

Aperçu rapide pour Hemoglobin Subunit beta-C (LOC100134870) (N-Term) anticorps (ABIN7565771)

Antigène: Hemoglobin Subunit beta-C (LOC100134870)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: ELISA, Western Blotting (WB)

Clone: 15C2-C11-F2-G11

Détail du produit

Épitope: N-Term

Fonction: Hemoglobin beta C Antibody

Réactivité croisée (Details): This protein A purified mouse monoclonal antibody reacts specifically with human HbC beta c-variant isoform.

Attributs du produit: Synonyms: mouse anti-HbC antibody, mouse anti-hemoglobin antibody, Hemoglobin beta subunit C variant, HbBc, HbC, HbC Antibody, LVV-hemorphin-7, Spinorphin, Beta-globin, Hemoglobin beta chain, Sickle Cell Disease (SCD)

Purification: Anti-HbC is purified from tissue culture supernatant by protein A purification.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Anti-Hemoglobin beta C Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near the N-terminus of Hb beta-subunit conjugated to KLH.

Immunogen Type: Conjugated Peptide

Isotype: IgG1 kappa

Information d'application

Indications d'application: Application Note: Anti-Hemoglobin beta C (MOUSE) antibody has been tested by ELISA and Western Blotting. This antibody is designed for use in lateral flow. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 16 kDa. Western Blot Dilution: 1 μg/mL ELISA Dilution: 1:20,000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: Hemoglobin Subunit beta-C (LOC100134870)

Autre désignation: HBBc

Sujet: Background: HbC antibodies detect the E6K mutant in the hemoglobin beta subunit. Functional hemoglobin (Hb) is a hetero tetramer composed of 2 alpha and 2 beta subunits (α2β2). Common isoform variants of hemoglobin include HbA, HbS, HbC, HbF, and HbA2. Sickle cell disease (SCD), thalassemias and hemoglobinopathies occur when aberrant forms of hemoglobin are expressed in children and adults. Globin gene mutations affect the structure and expression levels of Hb. Sickle cell disease and the more benign sickle cell trait are observed in more than 100 million people globally. Less significant than the SCD-E6V, HbC E6K mutation causes a mild hemolytic anemia. HbC antibody does not react to other forms of Hb. This antibody is ideal for investigators involved in Cardiovascular and developmental biology research.

UniProt: P68871