Protein G anticorps (FITC)

N° du produit ABIN7565828

L’anticorps Lapin Polyclonal anti-Protein G a été validé pour FACS, DB, WB, FM et FLISA. Il convient pour détecter Protein G dans des échantillons de Streptococcus.

Aperçu rapide pour Protein G anticorps (FITC) (ABIN7565828)

Antigène: Protein G

Reactivité: Streptococcus

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Protein G est conjugé à/à la FITC

Application: Flow Cytometry (FACS), Dot Blot (DB), Western Blotting (WB), Fluorescence Microscopy (FM), FLISA

Détail du produit anti-Protein G anticorps (FITC)

Fonction: Protein G Antibody Fluorescein Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum and Protein G.

Attributs du produit: Synonyms: rabbit anti-Protein G Antibody FITC Conjugation, Fluorescein conjugated rabbit anti-Protein G Antibody, Protein G FITC

Purification: Anti-Protein G antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Protein G (Streptococcus species)

Immunogen Type: Native Protein

Labeling Ratio: 4.5

Information d'application

Indications d'application: Application Note: FITC Conjugated Anti-Protein G antibody has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Flow Cytometry Dilution: User Optimized Western Blot Dilution: User Optimized FLISA Dilution: User Optimized IF Microscopy Dilution: 1:50 - 1:250 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détails sur Protein G

Antigène: Protein G

Sujet: Background: Protein G is a protein that has the property of binging to immunoglobulins. It is a 65- kDa cell surface protein that is commonly used for purifying antibodies through binding to the Fab and Fc regions. Protein G was originally isolated from Streptococcal bacteria. It is similar in properties to Protein A but has unique IgG binding specificities. Native protein G also binds albumin, however Rockland uses recombinant forms of Protein G that only bind to immunoglobulins.