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RFP anticorps

Cet anticorps Chèvre Polyclonal détecte spécifiquement RFP dans WB, ELISA et FM. Il présente une réactivité envers Discosoma.
N° du produit ABIN7565833

Aperçu rapide pour RFP anticorps (ABIN7565833)

Antigène

Voir toutes RFP Anticorps
RFP (Red Fluorescent Protein (RFP))

Reactivité

Discosoma

Hôte

  • 19
  • 16
  • 9
  • 6
  • 3
  • 1
Chèvre

Clonalité

  • 34
  • 20
Polyclonal

Conjugué

  • 28
  • 4
  • 3
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp RFP est non-conjugé

Application

  • 35
  • 22
  • 22
  • 13
  • 11
  • 11
  • 11
  • 10
  • 6
  • 4
  • 2
  • 1
  • 1
Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)
  • Fonction

    RFP Antibody

    Réactivité croisée (Details)

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Red Fluorescent Protein (Discosoma).

    Attributs du produit

    Synonyms: goat anti-RFP antibody, DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583

    Purification

    Anti-RFP (GOAT) is an IgG fraction purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

    Stérilité

    Sterile filtered

    Immunogène

    Immunogen: The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.

    Immunogen Type: Recombinant Protein

  • Indications d'application

    Application Note: Polyclonal anti-RFP has been tested by and western blot and is designed to detect RFP and its variants. Optimal titers for applications should be determined by the researcher. Expect a band ~27 kDa in Western Blot in the appropriate cell lysate or extract. Western Blot Dilution: 1:1000-1:10,000 ELISA Dilution: 1:2,000 - 1:10,000 IF Microscopy Dilution: User Optimized Other: User Optimized

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    Date de péremption

    12 months
  • Antigène

    RFP (Red Fluorescent Protein (RFP))

    Autre désignation

    DsRed

    Sujet

    Background: Antibodies to RFP (Discosoma spp.) are intended for use in immunological assays including ELISA, western blotting, immunofluorescence, and fluorescence activated cell sorting (FACS).

    UniProt

    Q9U6Y8
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