RFP anticorps

N° du produit ABIN7565834

L’anticorps Chèvre Polyclonal anti-RFP a été validé pour WB, ELISA et FM. Il convient pour détecter RFP dans des échantillons de Discosoma.

Aperçu rapide pour RFP anticorps (ABIN7565834)

Antigène: RFP (Red Fluorescent Protein (RFP))

Reactivité: Discosoma

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp RFP est non-conjugé

Application: Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)

Détail du produit anti-RFP anticorps

Fonction: RFP Antibody

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Red Fluorescent Protein (Discosoma).

Attributs du produit: Synonyms: goat anti-RFP antibody, DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583

Purification: Anti-RFP (GOAT) is an IgG fraction purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Stérilité: Sterile filtered

Immunogène:

Immunogen: The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.

Immunogen Type: Recombinant Protein

Information d'application

Indications d'application: Application Note: Polyclonal anti-RFP has been tested by western blot and is designed to detect RFP and its variants. Optimal titers for applications should be determined by the researcher. Expect a band ~27 kDa in Western Blot in the appropriate cell lysate or extract. Western Blot Dilution: 1:1000-1:10,000 ELISA Dilution: 1:2,000 - 1:10,000 IF Microscopy Dilution: User Optimized Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détails sur RFP

Antigène: RFP (Red Fluorescent Protein (RFP))

Autre désignation: DsRed

Sujet: Background: Antibodies to RFP (Discosoma spp.) are intended for use in immunological assays including ELISA, western blotting, immunofluorescence, and fluorescence activated cell sorting (FACS).

UniProt: Q9U6Y8