RFP anticorps

N° du produit ABIN7565836

Cet anticorps Souris Monoclonal détecte spécifiquement RFP dans WB, ELISA, IP, FACS et FM. Il présente une réactivité envers Discosoma.

Aperçu rapide pour RFP anticorps (ABIN7565836)

Antigène: RFP (Red Fluorescent Protein (RFP))

Reactivité: Discosoma

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp RFP est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Clone: 8E5-G7

Détail du produit anti-RFP anticorps

Fonction: RFP Antibody

Réactivité croisée (Details): Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry.

Attributs du produit: Synonyms: mouse anti-RFP Antibody, DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583

Purification: Anti-RFP Monoclonal Antibody was purified from concentrated tissue culture supernate by Protein A chromatography.

Stérilité: Sterile filtered

Immunogène:

Immunogen: The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full-length amino acid sequence (234aa) derived from the mushroom anemone Discosoma.

Immunogen Type: Recombinant Protein

Isotype: IgG2a kappa

Information d'application

Indications d'application: Application Note: Monoclonal anti-RFP is designed to detect RFP and its variants. This antibody has been tested by ELISA and Western blot, and is suitable for use in FISH, IF, IHC, and multiplex assays based on published references. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Optimal titers for applications should be determined by the researcher. Flow Cytometry Dilution: User Optimized Western Blot Dilution: 1:1,000 - 1:10,000 Immunoprecipitation Dilution: User Optimized ELISA Dilution: 1:75,000 - 1:150,000 IF Microscopy Dilution: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store Anti-RFP at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détails sur RFP

Antigène: RFP (Red Fluorescent Protein (RFP))

Autre désignation: DsRed

Sujet: Background: Antibodies to RFP (Discosoma spp.) are intended for use in immunological assays including ELISA, Western blotting, immunohistochemistry, and flow cytometry. RFP Proteins are useful markers for imagining protein localization, monitoring physiological processes, and detecting transgenic expression. Rockland's anti-RFP antibody can be used to detect native RFP and RFP variants.

UniProt: Q9U6Y8