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SOXA anticorps (HRP)

Cet anticorps anti-SOXA est un anticorps Chèvre Polyclonal détectant SOXA dans ELISA, IP et WB. Adapté pour Microbial.
N° du produit ABIN7565841

Aperçu rapide pour SOXA anticorps (HRP) (ABIN7565841)

Antigène

SOXA (SoxA Gene Product (SOXA))

Reactivité

Microbial

Hôte

Chèvre

Clonalité

Polyclonal

Conjugué

Cet anticorp SOXA est conjugé à/à la HRP

Application

ELISA, Immunoprecipitation (IP), Western Blotting (WB)
  • Fonction

    Sarcosine Oxidase Antibody Peroxidase Conjugated

    Réactivité croisée (Details)

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Sarcosine Oxidase [Microbial].

    Attributs du produit

    Synonyms: goat anti-Sarcosine Oxidase Antibody, HRP Conjugated goat anti-Sarcosine Oxidase Antibody, Sarcosine oxidase subunit alpha, Sarcosine oxidase subunit

    Purification

    Anti-Sarcosine Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

    Stérilité

    Sterile filtered

    Immunogène

    Immunogen: Sarcosine Oxidase [Microbial]

    Immunogen Type: Native Protein

  • Indications d'application

    Application Note: Anti-Sarcosine Oxidase has been tested by ELISA and western blot and is assayed against 1.0 μg of Sarcosine Oxidase [Microbial] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:3,000 of the reconstitution concentration is suggested for this product. Western Blot Dilution: 1:500 - 1:1,000 Immunoprecipitation Dilution: 1:100 ELISA Dilution: 1:1,000 - 1:2,500 Other: User Optimized

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1.0 mg/mL

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Stock

    -20 °C

    Stockage commentaire

    Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

    Date de péremption

    12 months
  • Antigène

    SOXA (SoxA Gene Product (SOXA))

    Autre désignation

    soxA

    Sujet

    Background: Sarcosine oxidase is an enzyme that catalyzes the oxidative demethylation of sarcosine to yield glycine, hydrogen peroxide, and 5,10-methylenetetrahydrofolate in a reaction requiring H4-tetrahydrofolate and oxygen.

    ID gène

    10586229

    NCBI Accession

    YP_004446819

    UniProt

    Q46337
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