TUBA1B anticorps (C-Term)

N° du produit ABIN7565852

L’anticorps Souris Monoclonal anti-TUBA1B a été validé pour WB, ELISA, IF, IHC, FM et MA. Il convient pour détecter TUBA1B dans des échantillons de Humain.

Aperçu rapide pour TUBA1B anticorps (C-Term) (ABIN7565852)

Antigène: TUBA1B (Tubulin, alpha 1B (TUBA1B))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TUBA1B est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Fluorescence Microscopy (FM), Multiplex Assay (MA)

Clone: 17H11-F10

Détail du produit anti-TUBA1B anticorps

Épitope: C-Term

Fonction: Alpha-Tubulin Antibody

Réactivité croisée (Details): This Loading Control antibody is directed against alpha tubulin.

Attributs du produit: Synonyms: mouse anti-Alpha-Tubulin Antibody, Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain, Alpha-tubulin ubiquitous, Tubulin K-alpha-1, TUBA1B, tubulin loading control

Purification: Anti-Tubulin Loading Control Antibody was purified by Protein A chromatography.

Stérilité: Sterile filtered

Immunogène: Anti-Tubulin Loading Control Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues near the C terminal end of human alpha tubulin protein.

Isotype: IgG1 kappa

Information d'application

Indications d'application: Application Note: Anti-Tubulin Antibody has been tested for use in ELISA, immunohistochemistry, immunofluorescence microscopy and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band at ~50 kDa in size corresponding to alpha tubulin by western blotting in most cell lysates or extracts. Immunohistochemistry Dilution: 2.5 μg/mL Western Blot Dilution: 1:1,000 ELISA Dilution: 1:300,000 IF Microscopy Dilution: 0.1 μg/mL Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détails sur TUBA1B

Antigène: TUBA1B (Tubulin, alpha 1B (TUBA1B))

Autre désignation: TUBA1B

Sujet: Background: Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle.  This antibody makes an excellent loading control.

ID gène: 17986283

UniProt: P68363

Pathways: Dynamique des Microtubules, M Phase