GLB1 anticorps (HRP)

N° du produit ABIN7565860

Cet anticorps Lapin Polyclonal détecte spécifiquement GLB1 dans WB, IHC et ELISA. Il présente une réactivité envers E. coli.

Aperçu rapide pour GLB1 anticorps (HRP) (ABIN7565860)

Antigène: GLB1 (Galactosidase, beta 1 (GLB1))

Reactivité: E. coli

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GLB1 est conjugé à/à la HRP

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Détail du produit anti-GLB1 anticorps (HRP)

Fonction: Beta Galactosidase Antibody Peroxidase Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, and anti-Rabbit Serum as well as purified and partially purified Beta Galactosidase [E.

Attributs du produit: Synonyms: rabbit anti-Beta Galactosidase Antibody Peroxidase Conjugation, HRP conjugated rabbit anti-Beta Galactosidase Antibody, rabbit anti-beta gal HRP conjugated antibody

Purification: Beta Galactosidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Beta Galactosidase (E.coli)

Immunogen Type: Native Protein

Information d'application

Indications d'application: Application Note: Anti-Beta Galactosidase Peroxidase Conjugated Antibody has been tested by ELISA and Western blot and is suitable for dot blot, immunofluorescence microscopy, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity. The antibody recognizes both frozen tissue sections, paraffin embedded tissue and 4 % paraformaldehyde fixed tissue for most immunohistochemical analysis. A 1:5,000 dilution has been reported to be successful for staining by immunoblot of beta-galactosidase fusion proteins after transfer using a semi-dry transfer apparatus.  A 1:1,500 dilution has been reported to detect beta-galactosidase in adult rat spinal cord tissue after infection with helper-dependent adenovirus expressing lacZ.  In this particular experiment, tissue was perfused with 4 % paraformaldehyde and cryostat-cut (35 μm) to produce free-floating sections.  A 1:5,000 dilution has been reported to be successful for staining of brain sections from transgenic mice expressing nuclear beta-galactosidase when assayed by immunofluorescence microscopy.  A 1:5,000 dilution has been reported for immunofluorescent staining of methanol fixed, devitellinized Drosophila embryos.  Although a wide range of conditions was reported to be effective, a 1:10,000 dilution was noted to show no background and to be suitable for double labeling experiments.  Optimal titers for other applications should be determined by the researcher. Immunohistochemistry Dilution: 1:250 Western Blot Dilution: 1:500 - 1:2,000 ELISA Dilution: 1:2,000 - 1:5,000 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détails sur GLB1

Antigène: GLB1 (Galactosidase, beta 1 (GLB1))

Autre désignation: Beta Galactosidase

Sujet: Background: β-galactosidase, (beta-gal or β-gal), is a hydrolase enzyme that catalyzes the hydrolysis of β-galactosides into monosaccharides. It may also cleave fucosides and arabinosides but with much lower efficiency. It is an essential enzyme in the human body. Deficiencies in the protein can result in galactosialidosis or Morquio B syndrome.

NCBI Accession: NP_414878

UniProt: P00722

Pathways: Glycosaminoglycan Metabolic Process