Serum anticorps (HRP)

N° du produit ABIN7565863

Cet anticorps Lapin Polyclonal détecte spécifiquement Serum dans ELISA, WB, IHC et DB. Il présente une réactivité avec des échantillons de Boeuf (Vache).

Aperçu rapide pour Serum anticorps (HRP) (ABIN7565863)

Antigène: Serum

Reactivité: Boeuf (Vache)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: HRP

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Dot Blot (DB)

Détail du produit

Fonction: Bovine Serum Antibody Peroxidase Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and anti-Peroxidase.

Attributs du produit: Synonyms: Rabbit anti-Bovine Serum Peroxidase Conjugated Antibody, Rabbit anti-Bovine Serum Antibody HRP Conjugation

Purification: Bovine serum is an IgG fraction antibody purified from polyspecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Bovine serum proteins

Immunogen Type: Native Protein

Information d'application

Indications d'application: Application Note: Anti-Bovine Serum peroxidase conjugate has been tested by dot blot and is suitable to be assayed against 1.0 μg of Bovine IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product. Immunohistochemistry Dilution: User Optimized Western Blot Dilution: User Optimized ELISA Dilution: User Optimized Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Polyethylene Glycol (PEG-8000)

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: Serum

Sujet: Background: Anti-Bovine serum antibody detects bovine serum proteins. Serum proteins are those proteins remaining in portion of plasma after coagulation of blood, during which process the plasma protein fibrinogen is converted to fibrin and remains behind in the clot. Anti-Bovine serum antibody is ideal for investigators involved in Cell Signaling, Cellular Biology and Signal Transduction research.