CA2 anticorps (HRP)

N° du produit ABIN7565866

Cet anticorps Lapin Polyclonal détecte spécifiquement CA2 dans WB, IHC et ELISA. Il présente une réactivité envers Humain.

Aperçu rapide pour CA2 anticorps (HRP) (ABIN7565866)

Antigène: CA2 (Carbonic Anhydrase II (CA2))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CA2 est conjugé à/à la HRP

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Détail du produit anti-CA2 anticorps (HRP)

Fonction: Carbonic Anhydrase II Antibody Peroxidase Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Carbonic Anhydrase II [Human Erythrocytes].

Attributs du produit: Synonyms: rabbit anti-Carbonic Anhydrase II Antibody Peroxidase Conjugation, HRP conjugated rabbit anti-Carbonic Anhydrase II Antibody, Carbonate dehydratase II antibody, Carbonic anhydrase 2 antibody, Carbonic anhydrase B antibody, Carbonic anhydrase C antibody, Carbonic anhydrase II antibody, Carbonic dehydratase antibody, CAC, CA-II

Purification: Carbonic anhydrase II is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Carbonic Anhydrase II [Human Erythrocytes]

Immunogen Type: Native Protein

Information d'application

Indications d'application: Application Note: Anti-Carbonic Anhydrase II Peroxidase Antibody has been tested by Western blot and suitable to be assayed against 1.0 μg of Carbonic Anhydrase II [Human Erythrocytes] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:6,000 of the reconstitution concentration is suggested for this product. Immunohistochemistry Dilution: 1:100 - 1:500 Western Blot Dilution: 1:250 - 1:1,000 ELISA Dilution: 1:500 - 1:2,500 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détails sur CA2

Antigène: CA2 (Carbonic Anhydrase II (CA2))

Autre désignation: CA2

Sujet: Background: Carbonic anhydrase II (CA2), is one of fourteen forms of human α carbonic anhydrases. Carbonic anhydrase catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Carbonic anhydrase II has been shown to interact with Band 3 and Sodium-hydrogen antiporter 1.

ID gène: 760

NCBI Accession: NP_000058

UniProt: P00918