CPA1 anticorps (HRP)

N° du produit ABIN7565867

L’anticorps Lapin Polyclonal anti-CPA1 a été validé pour WB, IHC et ELISA. Il convient pour détecter CPA1 dans des échantillons de Boeuf (Vache).

Aperçu rapide pour CPA1 anticorps (HRP) (ABIN7565867)

Antigène: CPA1 (Carboxypeptidase A1 (Pancreatic) (CPA1))

Reactivité: Boeuf (Vache)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CPA1 est conjugé à/à la HRP

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Détail du produit anti-CPA1 anticorps (HRP)

Fonction: Carboxypeptidase A Antibody Peroxidase Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Carboxypeptidase A [Bovine Pancreas].

Attributs du produit: Synonyms: rabbit anti-Carboxypeptidase A antibody peroxidase conjugation, HRP conjugated rabbit anti-Carboxypeptidase A Antibody, Carboxypeptidase A1 precursor antibody, CPA1 antibody, Pancreatic carboxypeptidase A1 antibody, Procarboxypeptidase A1 pancreatic antibody

Purification: Carboxypeptidase A is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Carboxypeptidase A [Bovine Pancreas]

Immunogen Type: Native Protein

Information d'application

Indications d'application: Application Note: Anti-Carboxypeptidase A Peroxidase Conjugated Antibody has been tested by ELISA and western blot and is useful in immunohistochemistry. Optimal titers should be obtained by researchers. Immunohistochemistry Dilution: User Optimized Western Blot Dilution: 1:300 - 1:2,000 ELISA Dilution: 1:3,000 - 1:10,000 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détails sur CPA1

Antigène: CPA1 (Carboxypeptidase A1 (Pancreatic) (CPA1))

Autre désignation: CPA1

Sujet: Background: Carboxypeptidase A (CPA-1) is produced in the pancreas and is crucial to many processes in the human body to include digestion, post-translational modification of proteins, blood clotting, and reproduction. There are two proposed mechanisms for the catalytic function of carboxypeptidase A. The first is a nucleophilic pathway involving a covalent acyl enzyme intermediate containing active site base Glu-270. The second proposed mechanism is a promoted water pathway. This mechanism involves attack of a water molecule at the scissile peptide linkage of the substrate.

ID gène: 286762

NCBI Accession: NP_777175

UniProt: P00730