PRC1 anticorps (HRP)

N° du produit ABIN7565868

Cet anticorps anti-PRC1 est un anticorps Lapin Polyclonal détectant PRC1 dans WB, ELISA et IHC. Adapté pour Saccharomyces cerevisiae.

Aperçu rapide pour PRC1 anticorps (HRP) (ABIN7565868)

Antigène: PRC1 (Protein Regulator of Cytokinesis 1 (PRC1))

Reactivité: Saccharomyces cerevisiae

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PRC1 est conjugé à/à la HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Détail du produit anti-PRC1 anticorps (HRP)

Fonction: Carboxypeptidase Y Antibody Peroxidase Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Carboxypeptidase Y [Baker's Yeast].

Attributs du produit: Synonyms: rabbit anti-Carboxypeptidase Y antibody peroxidase conjugation, HRP conjugated rabbit anti-Carboxypeptidase Y Antibody, Carboxypeptidase YSCY antibody, CPY1 antibody, LBC1 antibody, PRC1 antibody, Vacuolar carboxypeptidase Y antibody, YMR297W antibody, Carboxypeptidase C

Purification: Carboxypeptidase Y is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Carboxypeptidase Y [Baker's Yeast]

Immunogen Type: Native Protein

Information d'application

Indications d'application: Application Note: Anti-Carboxypeptidase Y Peroxidase antibody has been tested by ELISA and western blot and is assayed against 1.0 μg of Carboxypeptidase Y [Baker's Yeast] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product. Immunohistochemistry Dilution: User Optimized Western Blot Dilution: 1:1,000 - 1:5,000 ELISA Dilution: 1:2,000 - 1:10,000 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détails sur PRC1

Antigène: PRC1 (Protein Regulator of Cytokinesis 1 (PRC1))

Autre désignation: PRC1

Sujet: Background: Carboxypeptidase Y is an enzyme that catalyzes the reaction of the release of a C-terminal amino acid with broad specificity. Carboxypeptidase Y is a carboxypeptidase with optimum activity at pH 4.5-6.0. It is inhibited by diisopropyl fluorophosphate.

ID gène: 855343

NCBI Accession: NP_014026

UniProt: P00729