Zwf anticorps (HRP)

N° du produit ABIN7565882

L’anticorps anti-Zwf Polyclonal Chèvre est utilisé pour la détection de Zwf dans des échantillons de Leuconostoc mesenteroides. Il a été validé pour ELISA et WB.

Aperçu rapide pour Zwf anticorps (HRP) (ABIN7565882)

Antigène: Zwf

Reactivité: Leuconostoc mesenteroides

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: HRP

Application: ELISA, Western Blotting (WB)

Détail du produit

Fonction: Glucose-6-Phosphate Dehydrogenase Antibody Peroxidase Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Glucose-6-Phosphate-Dehydrogenase [Yeast].

Attributs du produit: Synonyms: goat anti-Glucose-6-Phosphate Dehydrogenase Antibody HRP Conjugation, Peroxidase Conjugated goat anti-Glucose-6-Phosphate Dehydrogenase Antibody, G6PD antibody, G6PD1 antibody, G6pdx antibody, Glucose 6 phosphate 1 dehydrogenase antibody, MET19 antibody, POS10 antibody, Zwf1p antibody

Purification: Glucose-6-Phosphate Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Glucose-6-Phosphate-Dehydrogenase [Yeast]

Immunogen Type: Native Protein

Information d'application

Indications d'application: Application Note: Anti-Glucose-6-Phosphate Dehydrogenase has been tested in ELISA and western blot. This product is assayed against 1.0 μg of Glucose-6-Phosphate-Dehydrogenase [Yeast] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product. Western Blot Dilution: 1:500 - 1:2,000 ELISA Dilution: 1:5,000 - 1:20,000 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: Zwf

Sujet: Background: Glucose-6-Phosphate Dehydrogenase is in the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells (such as erythrocytes) by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH), which maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. Cell growth and proliferation are affected by Glucose-6-Phosphate Dehydrogenase. Glucose-6-Phosphate Dehydrogenase inhibitors are under investigation to treat cancers and other conditions. DHEA is a Glucose-6-Phosphate Dehydrogenase inhibitor.

ID gène: 6063509

UniProt: P11411