Glycerol Kinase anticorps (HRP)

N° du produit ABIN7565884

Cet anticorps Chèvre Polyclonal détecte spécifiquement Glycerol Kinase dans ELISA et WB. Il présente une réactivité envers Cellulomonas.

Aperçu rapide pour Glycerol Kinase anticorps (HRP) (ABIN7565884)

Antigène: Glycerol Kinase (GK)

Reactivité: Cellulomonas

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Glycerol Kinase est conjugé à/à la HRP

Application: ELISA, Western Blotting (WB)

Détail du produit anti-Glycerol Kinase anticorps (HRP)

Fonction: Glycerol Kinase Antibody HRP Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Glycerol Kinase [Cellulomonas species].

Attributs du produit: Synonyms: goat anti-Glycerol Kinase Antibody HRP Conjugation, Peroxidase Conjugated goat anti-Glycerol Kinase Antibody, ATP glycerol 3 phosphotransferase antibody, GK antibody, GK antibody, GK1 antibody, GKD antibody, Glycerokinase antibody, Glycerol kinase antibody, Glycerol kinase deficiency antibody

Purification: Glycerol Kinase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Glycerol Kinase [Cellulomonas species]

Immunogen Type: Native Protein

Information d'application

Indications d'application: Application Note: Anti-Glycerol Kinase has been tested by ELISA and western blot. this product is assayed against 1.0 μg of Glycerol Kinase [Cellulomonas species] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:500 to 1:2,500 of the reconstitution concentration is suggested for this product. Western Blot Dilution: 1:500 - 1:2,500 ELISA Dilution: 1:1,000 - 1:5,000 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détails sur Glycerol Kinase

Antigène: Glycerol Kinase (GK)

Autre désignation: glpK

Sujet: Background: Glycerol Kinase is a key enzyme in the regulation of glycerol uptake and metabolism. Glycerol kinase is a phosphotransferase enzyme involved in triglycerides and glycerophospholipids synthesis.

ID gène: 6960048

UniProt: D0VZG4