ampC anticorps (HRP)

N° du produit ABIN7565904

Cet anticorps anti-ampC est un anticorps Lapin Polyclonal détectant ampC dans WB, IP et DB. Adapté pour Enterobacter cloacae.

Aperçu rapide pour ampC anticorps (HRP) (ABIN7565904)

Antigène: ampC (beta-Lactamase (ampC))

Reactivité: Enterobacter cloacae

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ampC est conjugé à/à la HRP

Application: Western Blotting (WB), Immunoprecipitation (IP), Dot Blot (DB)

Détail du produit anti-ampC anticorps (HRP)

Fonction: Penicillinase Antibody Peroxidase Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Penicillinase [Enterobacter cloacae].

Attributs du produit: Synonyms: rabbit anti-Penicillinase Antibody Peroxidase Conjugation, HRP Conjugated rabbit anti-Penicillinase Antibody, Aer1 antibody, Beta lactamase 1 antibody, Beta lactamase AER 1 antibody, OXA9 antibody, Oxacillinase antibody, PenPC antibody, Cephalosporinase

Purification: Penicillinase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Penicillinase [Enterobacter cloacae]

Immunogen Type: Native Protein

Information d'application

Indications d'application: Application Note: Anti-Penicillinase Peroxidase conjugated antibody has been tested by dot blot and is suitable to be assayed against 1.0 μg of Penicillinase [Enterobacter cloacae] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product. Western Blot Dilution: 1:250 Immunoprecipitation Dilution: 1:100 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détails sur ampC

Antigène: ampC (beta-Lactamase (ampC))

Autre désignation: ampC

Sujet: Background: Penicillinase is a specific type of β-lactamase, showing specificity for penicillin, again by hydrolyzing the beta-lactam ring. Molecular weights of the various penicillinases cluster around 50 kDa. Beta-lactamase provides antibiotic resistance by breaking the antibiotics' structure. These antibiotics all have a common element in their molecular structure: a four-atom ring known as a beta-lactam. Through hydrolysis, the lactamase enzyme breaks the β-lactam ring open, deactivating the molecule's antibacterial properties.

ID gène: 9125732

UniProt: P05364