Pepsin A anticorps (HRP)

N° du produit ABIN7565905

Cet anticorps Chèvre Polyclonal détecte spécifiquement Pepsin A dans WB, IHC, ELISA et DB. Il présente une réactivité envers Porc.

Aperçu rapide pour Pepsin A anticorps (HRP) (ABIN7565905)

Antigène: Pepsin A

Reactivité: Porc

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Pepsin A est conjugé à/à la HRP

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Dot Blot (DB)

Détail du produit anti-Pepsin A anticorps (HRP)

Fonction: Pepsin Antibody Peroxidase Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Pepsin [Porcine Stomach].

Attributs du produit: Synonyms: goat anti-Pepsin Antibody HRP Conjugated, Peroxidase Conjugated goat anti-Pepsin Antibody, Pepsin A antibody, Pepsin a precursor antibody, Pepsin antibody, Pepsinogen 1 second locus antibody, Pepsinogen 3 group 1 antibody, Pepsinogen a3 antibody

Purification: Pepsin is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Pepsin [Porcine Stomach]

Immunogen Type: Native Protein

Information d'application

Indications d'application: Application Note: Anti-Pepsin peroxidase has been tested by ELISA, dot blot, and western blot and is useful in immunohistochemistry. Optimal titers should be obtained by researchers if not provided. Immunohistochemistry Dilution: 1:250 - 1:1,250 Western Blot Dilution: 1:500 - 1:2,500 ELISA Dilution: 1:2,000 - 1:10,000 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détails sur Pepsin A

Antigène: Pepsin A

Autre désignation: PGA

Sujet: Background: Pepsin is one of three principal protein-degrading, or proteolytic, enzymes in the digestive system, the other two being chymotrypsin and trypsin. Pepsin is commonly used in the preparation produce either an Fab or an F(ab')2 fragment from antibodies.

ID gène: 396892

UniProt: P00791