MBP Tag anticorps (Biotin)

N° du produit ABIN7565908

Cet anticorps Lapin Polyclonal détecte spécifiquement MBP Tag dans WB et ELISA. Il présente une réactivité envers not_set.

Aperçu rapide pour MBP Tag anticorps (Biotin) (ABIN7565908)

Antigène: MBP Tag

Reactivité: Veuillez nous consulter SVP

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Biotin

Application: Western Blotting (WB), ELISA

Détail du produit

Fonction: Maltose Binding Protein (MBP) Epitope Tag Antibody Biotin Conjugated

Réactivité croisée (Details): This IgG purified antibody is directed against MBP and is useful in determining its presence in various assays. This polyclonal anti-MBP tag antibody detects over-expressed proteins containing the MBP epitope tag.

Attributs du produit: Synonyms: rabbit anti-MBP Epitope Tag Antibody biotin conjugation, rabbit anti-Maltose Binding Protein Antibody biotin conjugated

Purification: Purified antibody

Stérilité: Sterile filtered

Immunogène:

Immunogen: This antibody was purified from whole rabbit serum prepared by repeated immunizations with the MBP epitope tag recombinant protein.

Immunogen Type: Recombinant Protein

Information d'application

Indications d'application: Application Note: Anti-MBP Biotin Conjugated Antibody is optimally suited for monitoring the expression of MBP tagged fusion proteins.  As such, anti- MBP/MBP can be used to identify fusion proteins containing the MBP epitope.  The antibody recognizes the MBP epitope tag fused to the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against MBP containing recombinant proteins.  Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry, and other immunodetection techniques. Maltose binding protein is a bacterial protein, which is often used in protein expression studies because it creates a stable fusion product that does not appear to interfere with the bioactivity of the protein of interest. It also allows for its easy purification from bacterial extracts under mild conditions.  Anti-MBP is a companion to the pMAL protein expression system and can be used for the detection and purification of MBP-fusion proteins expressed in E. coli.  By Western blot, a band is seen at ~ 42 kDa representing MBP. Western Blot Dilution: 1:1,000-1:5,000 ELISA Dilution: 1:10,000-1:50,000 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: MBP Tag

Classe de substances: Tag

Sujet: Background: Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies.  Due to their small size, epitope tags do not affect the tagged protein's biochemical properties.   Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence.  This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means.  This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.  The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures.  Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.