HIST1H3A anticorps (C-Term)

N° du produit ABIN7565940

Cet anticorps anti-HIST1H3A est un anticorps Lapin Polyclonal détectant HIST1H3A dans ELISA, WB et IHC. Adapté pour Humain.

Aperçu rapide pour HIST1H3A anticorps (C-Term) (ABIN7565940)

Antigène: HIST1H3A (Histone Cluster 1, H3a (HIST1H3A))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp HIST1H3A est non-conjugé

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-HIST1H3A anticorps

Épitope: C-Term

Fonction: Histone H3 Antibody

Réactivité croisée (Details): Anti-Histone H3 is directed against the human histone 3 protein.

Attributs du produit: Synonyms: rabbit anti-histone H3 antibody, Histone H3.1, H3F, H3FA, H3FB, H3FL, H3FL, H3FC, H3FD, H3FI, H3FH, H3FK, H3FF, H3FJ, Histone 3 Antibody

Purification: The product was protein A purified from monospecific antiserum by immunoaffinity chromatography using protein A coupled to agarose beads.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Anti-Histone-3 was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to the c-terminus region of human histone-3.

Immunogen Type: Conjugated Peptide

Information d'application

Indications d'application: Application Note: Histone H3 antibody has been tested for use in ELISA and western blot. This antibody is suitable for IHC. For western blots expect a band of approximately 15.4 kDa in size corresponding to the Histone 3 protein. Specific conditions for reactivity should be optimized by the end user. Immunohistochemistry Dilution: User Optimized Western Blot Dilution: 1.0 μg/mL ELISA Dilution: 5.0 μg/mL Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.98 mg/mL

Buffer: Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store H3 antibody at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détails sur HIST1H3A

Antigène: HIST1H3A (Histone Cluster 1, H3a (HIST1H3A))

Autre désignation: HIST1H3A

Sujet: Background: Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Histone proteins are highly post-translationally modified with Histone H3 being the most extensively modified of the five histones. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H3 Antibody is ideal for investigators involved in Cell Signaling, Epigenetics, Nuclear Signaling research and Signal Transduction research.

ID gène: 8350

NCBI Accession: NP_003520

UniProt: P68431