CH25H anticorps (C-Term)

N° du produit ABIN7565948

L’anticorps Lapin Polyclonal anti-CH25H a été validé pour WB, ELISA et IHC. Il convient pour détecter CH25H dans des échantillons de Humain.

Aperçu rapide pour CH25H anticorps (C-Term) (ABIN7565948)

Antigène: CH25H (Cholesterol 25-Hydroxylase (CH25H))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CH25H est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Détail du produit anti-CH25H anticorps

Épitope: C-Term

Fonction: CH25H Antibody

Réactivité croisée (Details): This affinity purified antibody is directed against human CH25H.

Attributs du produit: Synonyms: Rabbit Anti-Cholesterol 25-Hydroxylase, Rabbit Anti-CH25H, Cholesterol 25-Monooxygenase, EC 1.14.99.38, H25OH, C25H

Purification: This affinity purified antibody is directed against human CH25H.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Anti-CH25H antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region of human CH25H conjugated to Keyhole Limpet Hemocyanin (KLH).

Immunogen Type: Conjugated Peptide

Information d'application

Indications d'application: Application Note: Anti-CH25H Antibody has been tested in ELISA, WB, and IHC.  Expect a band at ~31.7 kDa in western blot using appropriate tissues or lysates.  Positive control used: Human Kidney Tissues in Immunohistochemistry. Immunohistochemistry Dilution: 1:100 Western Blot Dilution: 1:1,000 ELISA Dilution: 1:10,000 - 1:50,000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.92 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur CH25H

Antigène: CH25H (Cholesterol 25-Hydroxylase (CH25H))

Autre désignation: CH25H

Sujet: Background: CH25H (Cholesterol 25-Hydroxylase catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes. CH25H plays a key role in cell positioning and movement in lymphoid tissues: 25-hydroxycholesterol is an intermediate in biosynthesis of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC), an oxysterol that acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for several lymphoid cells. It may play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. In testis, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation. Anti-CH25H Antibody is useful for researchers interested in Alzheimer's Disease, Neuroscience, and metabolism.

ID gène: 9023

NCBI Accession: NP_003947

UniProt: O95992