CH25H anticorps (C-Term)

N° du produit ABIN7565949

Cet anticorps anti-CH25H est un anticorps Lapin Polyclonal détectant CH25H dans WB, ELISA et IHC. Adapté pour Humain.

Aperçu rapide pour CH25H anticorps (C-Term) (ABIN7565949)

Antigène: CH25H (Cholesterol 25-Hydroxylase (CH25H))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CH25H est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Détail du produit anti-CH25H anticorps

Épitope: C-Term

Fonction: CH25H Antibody

Réactivité croisée (Details): This affinity purified antibody is directed against human CH25H.

Attributs du produit: Synonyms: Rabbit Anti-Cholesterol 25-Hydroxylase, Rabbit Anti-CH25H, Cholesterol 25-Monooxygenase, EC 1.14.99.38, H25OH, C25H

Purification: This affinity purified antibody is directed against human CH25H.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Anti-CH25H antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region of human CH25H conjugated to Keyhole Limpet Hemocyanin (KLH).

Immunogen Type: Conjugated Peptide

Information d'application

Indications d'application: Application Note: Anti-CH25H Antibody has been tested in ELISA, WB, and IHC.  Expect a band at ~31.7 kDa in western blot using appropriate tissues or lysates.  Positive control used: Human Kidney Tissues in Immunohistochemistry. Immunohistochemistry Dilution: 1:100 Western Blot Dilution: 1:1,000 ELISA Dilution: 1:10,000 - 1:50,000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.92 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détails sur CH25H

Antigène: CH25H (Cholesterol 25-Hydroxylase (CH25H))

Autre désignation: CH25H

Sujet: Background: CH25H (Cholesterol 25-Hydroxylase catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes. CH25H plays a key role in cell positioning and movement in lymphoid tissues: 25-hydroxycholesterol is an intermediate in biosynthesis of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC), an oxysterol that acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for several lymphoid cells. It may play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. In testis, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation. Anti-CH25H Antibody is useful for researchers interested in Alzheimer's Disease, Neuroscience, and metabolism.

ID gène: 9023

NCBI Accession: NP_003947

UniProt: O95992