POLR2A/RPB1 anticorps (N-Term)

N° du produit ABIN7565953

Cet anticorps Lapin Polyclonal détecte spécifiquement POLR2A/RPB1 dans WB, ELISA, ChIP et FM. Il présente une réactivité envers Humain.

Aperçu rapide pour POLR2A/RPB1 anticorps (N-Term) (ABIN7565953)

Antigène: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp POLR2A/RPB1 est non-conjugé

Application: Western Blotting (WB), ELISA, Chromatin Immunoprecipitation (ChIP), Fluorescence Microscopy (FM)

Détail du produit anti-POLR2A/RPB1 anticorps

Épitope: N-Term

Fonction: POL II Antibody

Réactivité croisée (Details): This affinity purified antibody is directed against human POL II.

Attributs du produit: Synonyms: RPO2, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1, RPB1, POLR2A,

Purification: This affinity purified antibody is directed against human POL II.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Anti-POL II antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the n-terminal portion of human POL II conjugated to Keyhole Limpet Hemocyanin (KLH).

Immunogen Type: Conjugated Peptide

Information d'application

Indications d'application: Application Note: Anti-POL II antibody has been tested in ChIP and ELISA, and is likely suitable for western blotting and immunofluorescence assays. Positive controls used in ChIP were EGF stimulated NR4A1 5', NR4A1 3' and FOS 3'. The negative control was total rabbit IgG. ChIP Dilution: User Optimized Western Blot Dilution: 1:1000 ELISA Dilution: 1:10,000 - 1:50,000 IF Microscopy Dilution: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détails sur POLR2A/RPB1

Antigène: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Autre désignation: POLR2A

Sujet: Background: Anti-POL II antibody will detect DNA-directed RNA polymerase II subunit RPB1, an enzyme composed of 12 subunits, which initiates the transcription of RNA from DNA, synthesizing many functional non-coding RNAs as well as messenger RNA precursors. POL II encodes a core element of the transcription machinery. Malfunctioning of the Pol II gene can cause diseases such as Cockayne Syndrome B and La Crosse Encephalitis. Anti-POL II Antibody is useful for researchers interested in DNA damage and repair studies.

ID gène: 5430

NCBI Accession: NP_000928

UniProt: P24928

Pathways: Regulatory RNA Pathways