Protein A anticorps

N° du produit ABIN7565957

L’anticorps Poulet Polyclonal anti-Protein A a été validé pour ELISA, WB et DB. Il convient pour détecter Protein A dans des échantillons de Staphylococcus aureus.

Aperçu rapide pour Protein A anticorps (ABIN7565957)

Antigène: Protein A

Reactivité: Staphylococcus aureus

Hôte: Poulet

Clonalité: Polyclonal

Conjugué: Cet anticorp Protein A est non-conjugé

Application: ELISA, Western Blotting (WB), Dot Blot (DB)

Détail du produit anti-Protein A anticorps

Fonction: Protein A Antibody

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Protein A [Staphylococcus aureus] and anti-Chicken Serum.

Attributs du produit: Synonyms: chicken anti-Protein A Antibody, chicken anti Protein A, chicken anti PA

Purification: This Anti-Protein A Antibody is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above.

Stérilité: Sterile filtered

Immunogène:

Immunogen: This IgY fraction antibody was prepared from eggs of chickens laid after repeated immunizations with Protein A  [Staphylococcus aureus].

Immunogen Type: Native Protein

Information d'application

Indications d'application: Application Note: Anti-PROTEIN A (CHICKEN) Antibody has been tested by western blotting and suitable for ELISA. Although not tested, this antibody may also be useful for neutralization assays, immunohistochemistry, flow Cytometry, immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring high titer and specificity. Western Blot Dilution: 1:500 - 1:2,000 ELISA Dilution: 1:500 - 1:2,000 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détails sur Protein A

Antigène: Protein A

Sujet: Background: Protein A is 56 kDA surface protein found in the cell wall of Staphylococcus aureus. Protein A has a high affinity to Human IgG, Mouse IgG and others and is therefore suitable for purification and detection of immunoglobulins.