F11R anticorps (pTyr280)

N° du produit ABIN7565976

Cet anticorps anti-F11R est un anticorps Lapin Polyclonal détectant F11R dans WB, ELISA, IF et FM. Adapté pour Humain.

Aperçu rapide pour F11R anticorps (pTyr280) (ABIN7565976)

Antigène: F11R (F11 Receptor (F11R))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp F11R est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM)

Détail du produit anti-F11R anticorps

Épitope: pTyr280

Fonction: JAM A phospho Y280 Antibody

Réactivité croisée (Details): Anti-JAM A pY280 is directed against human JAM A phosphorylated at the tyrosine 280 position.

Attributs du produit: Synonyms: rabbit anti-JAM A pY280 antibody, JAM-A, Junctional adhesion molecule A, JAM-1, Junctional adhesion molecule 1, Platelet F11 receptor, Platelet adhesion molecule 1, PAM-1, CD321, JAM1, JCAM, JAM 1, JAMA

Purification: This product is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide to remove any unwanted reactivities.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Affinity purified Anti-JAM A pY280 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the c-term and phosphorylated at the tyrosine 280 position of Human JAM A protein.

Immunogen Type: Conjugated Peptide

Information d'application

Indications d'application: Application Note: This affinity purified antibody has been tested for use in ELISA, IF, and western blot.   Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 32.5 kDa in size corresponding to JAM A by western blotting in the appropriate cell lysate or extract. Western Blot Dilution: 1 μg/mL ELISA Dilution: 5 μg/mL IF Microscopy Dilution: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détails sur F11R

Antigène: F11R (F11 Receptor (F11R))

Autre désignation: F11R

Sujet: Background: Anti-JAM A pY280 was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). JAM A pY280 Antibody detects junctional adhesion molecule A (JAM A) protein phosphorylated at the tyrosine 280 location. JAM A is involved in cell-cell contact and tight junction formation in epithelial cells. JAM A protein is a transmembrane protein expressed in epithelial and endothelial cells as well as in hematopoietic cells and is important for a variety of cellular processes including tight junction assembly, leukocyte transmigration, platelet activation, angiogenesis and virus binding. In addition, JAM A may be a potential therapeutic target for breast cancer.

ID gène: 50848

NCBI Accession: NP_058642

Pathways: Cell-Cell Junction Organization