RFP anticorps

N° du produit ABIN7565987

N° du produit (Fournisseur): 600-401-379

L’anticorps Lapin Polyclonal anti-RFP a été validé pour WB, ELISA, IF, IHC, IP, FACS et FM. Il convient pour détecter RFP dans des échantillons de Discosoma.

Aperçu rapide pour RFP anticorps (ABIN7565987)

Antigène: RFP (Red Fluorescent Protein (RFP))

Reactivité: Discosoma

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RFP est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Détail du produit anti-RFP anticorps

N° du produit (Fournisseur): 600-401-379

Fournisseur: Rockland

Fonction: RFP Antibody Pre-adsorbed

Réactivité croisée (Details): Minimal crossreactivity against Hu Ms & Rt Serum Proteins
Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry.

Attributs du produit: Synonyms: rabbit anti-RFP antibody, DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583.

Purification:

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Preadsorption: Pre-Adsorbed

Stérilité: Sterile filtered

Immunogène:

Immunogen: The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full-length amino acid sequence (234aa) derived from the mushroom anemone Discosoma.

Immunogen Type: Recombinant Protein

Information d'application

Indications d'application: Application Note: RFP Antibody Pre-adsorbed is designed for detecting RFP and its variants, tested across ELISA, Western blot, IF, IHC, and suitable for EM, FC, FISH, IP, and multiplex assays. It is applicable for ELISA in both sandwich and capture formats. Researchers should determine optimal titers for specific applications. Recommended starting dilutions are: ELISA 1:28,700 - 1:48,700, FC, IF, IHC 1:200 - 1:2,000, WB 1:1,000 - 1:5,000. Flow Cytometry Dilution: 1:200 - 1:2,000 Immunohistochemistry Dilution: 1:200 - 1:2,000 Western Blot Dilution: 1:1,000 - 1:5,000 Immunoprecipitation Dilution: User Optimized ELISA Dilution: 1:28,700 - 1:48,700 IF Microscopy Dilution: 1:200 - 1:2,000 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.17 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur RFP

Antigène: RFP (Red Fluorescent Protein (RFP))

Autre désignation: DsRed

Sujet: Background: RFP Antibody Pre-adsorbed is a high-quality, rabbit polyclonal antibody specifically designed for the detection of red fluorescent protein (RFP) and its variants like mCherry, mScarlet, and tdTomato. This product is suitable for a broad range of applications including Western blot (WB), ELISA, immunohistochemistry (IHC), immunofluorescence (IF), flow cytometry (FC), and more. It is uniquely pre-adsorbed to minimize cross-reactivity, offering high specificity and sensitivity. With over 900 references, this product stands as a proven tool in the scientific community, supporting a wide range of research studies from cellular signaling to disease pathology.

UniProt: Q9U6Y8